66 
HALLY JOLIVETTE SAX 
Figure 8 represents a section of the ascus containing one spindle 
figure of the fourth nuclear division in the ascus. It is typical of all 
of the spindle figures present. The spindle with the chromosomes in 
the center stains densely. The nuclear cavity is still distinct. The 
central bodies at the poles of the spindle stand out clearly. Numerous 
delicate fibers radiate out from the central body. These are very 
long and remarkably distinct which is characteristic of the well-stained 
figures. The fibers, however, cannot be seen as such except when 
they lie in the plane of the section. They are extremely delicate, but 
the large numbers seen in the plane of this figure render them rather 
conspicuous. 
In the sixteen-nucleate stage the nuclei in their successive divisions 
have gradually scattered through a larger part of the ascus, with the 
upper and lower portions, however, still containing only cytoplasm. 
The region of dense cytoplasm surrounding the nuclei is even more 
distinct than in the earlier divisions. From these denser regions 
strands of cytoplasm radiate outward in different directions. Figure 9 
represents a longitudinal section of the ascus at the sixteen-nucleate 
stage. Eight nuclei are visible in the section. At this stage the nuclei 
appear much the same as after the previous divisions, except that 
they are smaller. The nuclei do not increase much in size after 
dividing. Their structure is very similar and they are not beaked. 
The nuclei continue to divide rapidly until there are approximately 
128 in number. The later divisions are essentially like the earlier 
ones. The spindles are small and with distinct centers at each pole. 
The astral rays, which are strongly developed, radiate out from the 
centers. These divisions take place near the periphery of the ascus. 
Figure 10 represents a portion of the ascus containing a nucleus in 
the equatorial plate stage. This is one of the late divisions. The 
spindle is very small but clear, with a distinct central body at each 
end. The chromosomes are plainly differentiated in the center of 
the spindle. The nuclear cavity is still apparent. The clearness 
with which this figure shows up in so small a nucleus is remarkable. 
The asters are present at the poles of the spindle. The astral rays 
can be seen extending outward from the central bodies into the cyto- 
plasm. The fibers are extremely delicate, but apparent. The dense 
fine granular appearance of the remaining cytoplasm near the central 
body is probably due to the fact that numerous fibers have been 
cut in sectioning. 
