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HALLY JOLIVETTE SAX 
their final extrusion from the ascus. The long period of existence of 
such a large amount of epiplasm without nuclei is at least suggestive. 
It is certain from the study of Philocopra coeruleotecta that the 
spore wall is laid down by deposition from the outer layer of the 
cytoplasm within the spore and not by the epiplasm. The spore 
wall is as thick between the hyaline tail and the main portion of the 
spore as in the rest of the spore, where it is in contact with the epi- 
plasm. The cytoplasm of the spore has doubtless laid down that 
portion of the spore wall and probably the remaining part. 
The study of the stages in the development of the ascus and in 
spore formation shows the central body to be present at every stage. 
The sections were not always stained to bring out a well-defined 
central body, but in the most favorable preparations the central 
body stained differently from the chromatin material. It is very small 
but well defined. In the resting nuclei it appears as a small deep red- 
staining body in contact with the nuclear membrane. As a rule it 
is to be found on the side of the nucleus away from the nucleole. The 
central body is especially well defined in the division figures where the 
chromatin particles are more remote from it. They are found at 
the poles of the spindle on the sides of the nuclear cavity. The central 
bodies are so apparent that there is no doubt as to their existence. 
The definiteness of the spindle with the central bodies, even in the 
late divisions, is too remarkable to leave unmentioned. The minute 
figures are as clear and distinct as the larger figures. This suggests 
the possibility of finding more clearly defined figures in some of the 
other fungi, where they are not sufficiently distinct in the preparations 
so far figured. 
The astral rays can be seen radiating outward from the centers 
into the cytoplasm in different directions. Some of the preparations 
are not so favorable as others, but in good clear preparations the rays 
stand out distinctly. They are very fine, delicate, and numerous. 
In the fourth nuclear division (fig. 8) a large number of rays are seen 
in the plane in which the figure is drawn. Figures 6 and lo show 
clearly defined asters at the poles of the spindle. Not so many rays 
are seen in the plane of the figures, but the asters at the poles are 
unmistakable. There are a number of rays present and the cytoplasm 
in the immediate vicinity of the central body is fine and dense. The 
late divisions with a large number of nuclei show conspicuous asters. 
At the close of the last division the nuclei are beaked with the 
