Il8 CHAS. E. SANDO AND H. H. BARTLETT 
of colors. Ferric chloride gives a brownish-black color. The myri- 
cetin is darker in color than fisetin, and shows about the same solu- 
bilities; it differs in being slightly soluble in chloroform and only 
very slightly in acetic acid. 
The largest sample of myricetin was obtained from leaves of 
Rhus glabra. The acetyl myricetin from this source yielded 55.00 
percent of myricetin on hydrolysis. Theory requires 55.79 percent 
for Ci5H408(C2H30)6, the formula of the compound. For C15H5O8- 
(C2H30)5 and Ci5H308(C2H30)7 the yields would have been 60.22 per- 
cent and 51.96 percent respectively. Two combustions of the acetyl 
myricetin and one of myricetin were carried out, with results shown 
in Table III. The acetyl myricetin melted at 208-209° C. (un- 
corrected) when slowly heated. Perkin gives the melting point as 
211-212° C. 
Table III 
Combustions of Myricetin from Green Leaves of Rhus glabra {Sample A) and of its 
Acetyl Derivative {Samples B and C) 
Sample 
Weight 
CO2 
H2O 
H/i 
o,i 
A 
.0800 
.1656 
.0290 
56.45 
4.06 
39.49 
56.60 
3.14 
40.26 
B 
.0960 
.2012 
.0360 
57.15 
4.20 
38.65 
C 
•1332 
.2784 
.0510 
57-OI 
4.27 
38.72 
57.08 
4.24 
38.68 
Required for acetyl myricetin, 
Ci5H408(C2H30)6 
56.84 
3.86 
39.20 
Myricetin both glucosidal, and, in very slight traces, free, was 
also obtained from the red autumn leaves of R. glabra, and from the 
red berries. In the latter case the myricetin was free, but it may 
have been derived by hydrolysis from a glucoside, since the berries 
when boiled in water yield a strongly acid solution. Two samples 
of green leaves of Rhus copallina, kindly furnished by Dr. W. W. 
Stockberger, of the Bureau of Plant Industry, also proved to contain 
myricetin. They were collected by C. R. Gilmore at Muskogee, 
Oklahoma. Several attempts to isolate a flavone from the leaves of 
R. typhina, collected at Ann Arbor, were unsuccessful, although various 
methods were used and the operations were conducted on a large scale. 
Summary 
By the isolation of flavone pigments from three species of Rhus, 
R. typhina, R. glabra, and R. copallina, we have been able to verify 
