Oct., 1922] YOUNG AND BENNETT FUNGI IN CULTURE MEDIA 465 
ments for an optimum synthetic solution would be composed of the six 
elements, nitrogen, phosphorus, sulphur, potassium, calcium, and mag- 
nesium. 
We have no evidence to the effect that we have been using these inor- 
ganic elements in correct proportions. Since the salt requirements for 
fungi are probably the same as for higher plants, a series of culture solutions 
was arranged according to the method used by the National Research 
Council for work in the study of nutrition in higher plants. A triplicate 
series of twenty-one cultures, using potassium acid phosphate (monobasic), 
calcium nitrate, and magnesium sulphate, was arranged. The salts varied 
in the different solutions by increments of one eighth and had an osmotic 
concentration of three and one half atmospheres. Sucrose was added in 
equal amounts of 3.43 grams per 100 cc. of the solution, thereby giving the 
culture solutions a total osmotic concentration of four and one half at- 
mospheres. Similarly, a triplicate series was arranged, using potassium 
nitrate, calcium acid phosphate, and magnesium sulphate. 
Table 3 
Cc. of 
Cc. of 
Cc. of 
Cc. of 
Cc. of 
Sugar 
Water to 
Cc. of 
Cc. of 
Cc. of 
Sugar 
Water to 
No. 
M/14 
M/14 
M/14 
Sol.. 
make 
M/3.5 
M/3S Ca- 
M/3.5 
Sol., 
make 
KH2PO4 
Ca(N03)2 
MgS04 
.3-43 g- 
in 10 cc. 
50 cc. 
KNO3 
(H2P04)2 
MgS04 
3.4 g- 
in 10 cc. 
50 cc. 
II 
4 
4 
22.4 
5 
14.6 
.95 
9-5 
5-7 
5 
28.85 
12 
3-5 
6.8 
17.2 
5 
17-5 
.91 
18.6 
4.6 
5 
20.89 
13 
3-4 
10 
13.2 
5 
18.4 
.84 
25.6 
3-43 
5 
15-13 
14 
3-1 
12.4 
9-4 
5 
20.1 
.81 
32.6 
2.45 
5 
9.14 
15 
3.1 
15 
6 
5 
20.9 
•74 
37^11 
1.47 
5 
5-68 
16 
3-0 
17 
2.8 
5 
22.2 
•73 
43^75 
•74 
5 
.00 
21 
7-4 
4 
17.2 
5 
16.4 
1.89 
9.5 
4.62 
5 
29.03 
22 
7.0 
6.8 
13.2 
5 
18.0 
1.68 
18.6 
3-43 
5 
21.39 
23 
6.5 
10. 0 
9.4 
5 
19.6 
1.58 
25.6 
2.45 
5 
17.27 
24 
6.2 
12.4 
6.0 
5 
20.4 
1.47 
32.6 
1.47 
5 
9.46 
25 
6.0 
15.0 
2.8 
5 
21.2 
1.44 
37-1 
.74 
5 
5-72 
31 
10.6 
4.0 
13.2 
5 
17.2 
2.63 
9^5 
3-43 
5 
29.44 
32 
10,0 
6.8 
9.4 
5 
18.8 
2.45 
18.6 
2.45 
5 
21.50 
33 
9-5 
10. 0 
6.0 
5 
19-5 
2.35 
25.6 
1.47 
5 
15-58 
34 
9.0 
12.4 
2.8 
5 
20.8 
2.24 
32.6 
•74 
5 
9-42 
41 
13.8 
4.0 
9-4 
5 
17.8 
3-47 
9.5 
2.45 
5 
29.58 
42 
13.2 
6.8 
6.0 
5 
19.0 
3-25 
18.6 
1.47 
5 
21.68 
43 
12.6 
10. 0 
2.8 
5 
19.6 
2.98 
25.6 
■74 
5 
15-68 
51 
17.2 
4.0 
6.0 
5 
17.8 
4-38 
9.5 
1.74 
5 
29.65 
52 
16.4 
6.8 
2.8 
5 
19.0 
3-96 
18.6 
•74 
5 
21.70 
61 
20.3 
4.0 
2.8 
5 
17.9 
4.87 
9.5 
•74 
5 
29.89 
Osmotic concentration, 4.5 atm.; pH, 4.8-5.2. 
The method of arranging the concentrations is given in table 3. The 
inorganic salts used were of the highest purity of the grade mentioned 
above. The technique used was the same as in the preceding experiments. 
Special care was taken in inoculation of solutions so as to obtain uniform 
growths at the outset. Fusarium oxysporum, Macrosporium sarcinaeforme, 
and Phoma apiicola were used. Richards', Pfeffer's, Currie's, Meyer's, 
and Czapek's solutions were used as checks. 
