Oct., 1922] YOUNG AND BENNETT FUNGI IN CULTURE MEDIA 
469 
3. Calcium, in the case of the organisms used, proved to be beneficial 
in the majority of instances and seems to have a place, probably in the 
correcting of acidity, in the best synthetic solutions. 
4. The inorganic elements in a synthetic solution should be properly 
balanced. The balancing of these salts is a comparatively simple procedure 
when the three-salt or triangular system is used. By this system the 
mineral requirements of any particular fungus can be quickly and accurately 
ascertained. By then selecting the sugar most readily used, a favorable 
culture solution can be made. 
BIBLIOGRAPHY 
1. Bess^y, E. A. Ueber die Bedingungen der Farbbildung bei Fusarium. Flora 93: 301- 
344. 1904. 
2. CoonvS, G. H. Factors involved in the growth and pycnidium production of Plenodomus 
fuscomaculans . Jour. Agr. Res. 5: 713-769. 1916. 
3. Currie, J. N. The citric acid fermentation of Aspergillus niger. Jour. Biol. Chem. 
31:15. 1917. 
4. Dox, A. W. The intracellular enzymes of Penicillium and Aspergillus. U. S. Dept. 
Agr. Bur. Anim. Ind. Bull. 120: 1-70. 1910. 
5. Duggar, B. M. Fungous Diseases of Plants, p. 26. 1909. 
6. Graves, A. H. Chemotropism in Rhizopus nigricans. Bot. Gaz. 52: 337-370. 1916. 
7. Mayer, A. Untersuchungen iiber die alkoholische Gahrung, den Stofifbedarf und den 
Stoffwechsel der Hefepflanze. Heidelberg, 1879. 
8. Milburn, T. Ueber Aenderungen der Farben bei Pilzen und Bakterien. Centralbl. 
Bakt. II, 13: 258-276. 1904. 
9. Molisch, H. Die mineralische Nahrung der niederen Pilze. Bot. Centralbl. 60: 167, 
168. 1894. 
10. Pasteur, L. Memoire sur la fermentation alcoolique. Ann. Chem. Phys. Ill, 58: 
323. i860. 
11. Pfeffer, W. Ueber Election organischer Nahrstoffe. Jahrb. Wiss. Bot. 28: 205-268. 
1895. 
12. Raulm, J. Etudes chimiques sur la vegetation. Ann. Sci. Nat. Bot. V, 11: 93-299. 
1869. 
• 13. Richards, H. M. Die Beeinflussung des Wachstums einiger Pilze durch chemische 
Reize. Jahrb. Wiss. Bot. 30: 665-688. 1897. 
14. Steinberg, R. A. A study of some factors in the chemical stimulation of the growth of 
Aspergillus niger. Amer. Jour. Bot. 6: 330-372. 1919. 
15. Stevens, F. L., and Hall, J. G. Variation of fungi due to environment. Ann. Rept. 
N. C. Agr. Exp. Sta. (1909) 32: 47-71. 191 1. 
