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AMERICAN JOURNAL OF BOTANY 
[Vol. 9, 
development. Extremes were avoided, and it is probably due to the care 
exercised that injuries such as those reported by Frank and Kriiger (1894) 
were not observed. These authors reported a severe Bordeaux injury to 
the foliage of potatoes as the result of adverse conditions, including very 
high temperatures. Bain (1902) also states that high temperature is a 
factor in the physiological response of peach foliage to copper salts, being 
manifested in both increased assimilation and increased injury. Crandall 
(1909) states that low vitality is a factor in the production of injury to the 
foliage of the apple following the application of Bordeaux mixture. The 
freedom of the plants from injury may have been due in part to the fact 
that the foliage was not wetted, which, it is frequently stated, is a necessary 
condition, although Schander (1904) has shown that bean foliage can be 
injured by Bordeaux mixture without the presence of liquid water. Under 
conditions brought about by too low temperatures or too little illumination 
it is possible that the leaves of the bean would show no appreciable response. 
Summary 
1. The primordial leaves of the bean sprayed with Bordeaux mixture do 
not grow to the size attained by unsprayed leaves. 
2. A retardation in growth is manifested very soon after the spray is 
applied. The relative difference is maintained. 
3. The chlorophyll content per unit area of the primordial leaves of the 
bean is slightly increased by spraying with Bordeaux mixture. 
4. The chlorophyll content per unit area of the primordial leaves of the 
bean decreases as the leaves develop after the shedding of the cotyledons. 
5. The chlorophyll content per unit area of the young primordial leaves 
of the bean is lower than that of the primordial leaves of the bean just 
after the shedding of the cotyledons. 
6. The primordial leaves of the bean increase in area and in weight per 
unit area following the suppression of the growing buds. 
7. This increase in area and weight per unit area is accompanied by a 
disproportionately greater increase in the chlorophyll content per unit area. 
BIBLIOGRAPHY 
Amos, A. 1907. The effect of fungicides upon the assimilation of carbon dioxide by green 
leaves. Jour. Agr. Sci. 2: 257-266. 
Bain, S. M. 1902. The action of copper on leaves. Tenn. Exp. Sta. Bull. 15^: 1-108. 
Bell, J. M., and Taber, W. C. 1907. The action of lime in excess on copper sulphate 
solutions. Jour. Phys. Chem. 11: 632-638. 
Boussingaxilt, J. 1878. Etude sur les fonctions physiques des feuilles. Annales Chim. 
Phys. V, 13: 289-394. 
Clark, J. F. 1902. On the toxic properties of some copper compounds with special refer- 
ence to Bordeaux mixture. Bot. Gaz. 33: 26-48. 
Coupin, H. 1898. Sur la toxicite du chlorure de sodium et de I'eau de mer e I'egard des 
vegetaux. Rev. Gen. Bot. 10: 177-190. 
Crandall, C. S. 1909. 111. Exp. Sta. Bull. 135. 
