EFFECTS ON CHESTNUTS OF SUBSTANCES INJECTED 
53 
The ammonium compounds 1/500 Formic acid i/iooo G.M. 
G.M. Citric acid 1/50, 1/500 G.M. 
Apparently a slight stimulant 
The weaker dilutions of the alkali 
metals 
Para-nitro-phenol i/iooo G.M. 
Picric acid i/ioooo G.M. 
Slightly detrimental {blotched leaves, 
death of cambium near the point 
of injection) 
Para-nitro-phenol 1/500 G.M. 
Ortho-nitro-phenol i/iooo G.M. 
Picric acid 1/500, i/iooo G.M. 
Pyrocatechin i/iooo G.M. 
Pyrogallic acid i/iooo, 1/500 G.M. 
Phloroglucine i/iopo G.M. 
Benzoic acid 1/500 G.M. 
Phenol i/iooo, 1/500 G.M. 
Copper sulphate i/ioo G.M. 
Lithium salts i/ioo G.M. 
Ammonium compounds i/ioo G.M. 
Sodium chloride i/ioo G.M. 
Eosin 1/40 percent 
Methylene blue 1/40 percent 
Acetic acid i/iooo G.M. 
Summary 
For four years observations have been made on the effect of chemical 
solutions injected into the trunks of chestnut trees. 
1. Usually it was found that the visible effect of a solution on a tree 
varied with the distance from the point of injection. 
2. The effect varied with the dilution of the solution and the month in 
which the injection was made. 
3. In general the effect of the injection of the alkali metals was not 
detrimental to the trees; injection of heavy metals was detrimental; col- 
loidal metals were not detrimental; organic compounds were not detri- 
mental; water extract of chestnut blight canker was detrimental, healthy 
bark extract was not. 
4. Many of the bases produced characteristic discoloration of the leaves. 
5. Lithium was found in the nuts gathered from lithium-injected trees. 
The nuts gathered from the remaining trees were not tested sufficiently to 
show positively whether or not they contained any of the injected chemicals. 
Detrimental {death of injected part of 
tree or of whole tree) 
Copper sulphate 1/20 G.M. 
Copper chloride 1/20 G.M. 
Zinc carbonate 1/20 G.M. 
Mercuric chloride i/iooo G.M. 
Potassium chromate i/iooo, i/ioooo 
G.M. 
Potassium bichromate i/iooo, 
i/ioooo G.M. 
Barium chloride 1/20 G.M. 
Alkali metals 1/20 G.M. (NaCl 1/50 
G.M.) 
Formalin 0.4 percent 
Acetic acid i/ioo G.M. 
Formic acid i/ioo G.M. 
Lactic acid i/ioo G.M. 
Anilin sulphate i/ioo G.M. 
Meta-cresol i/iooo G.M. 
Benzoic acid 1/500 G.M. 
Salicylic acid i/ioo G.M. 
Water extract of chestnut blight 
canker 
