THE INJECTION OF CHEMICALS INTO CHESTNUT TREES 
9 
Sodium chloride 
Sodium hydroxide 
Lithium carbonate 
Lithium chloride 
Lithium sulphate 
Lithium hydroxide 
Lithium nitrate 
Water 
Phloroglucin 
Oil of bitter almonds 
Benzoic acid 
Salicylic acid 
Bark extracts 
Water extract of chestnut tree bark 
Water extract of chestnut blight 
canker 
Stains : 
Methyl green 
Methylene blue 
Eosin 
Congo red 
Trypan blue 
All these substances went into the trees in measurable quantities. 
Solutions 
The solutions were made gram molecular except in the case of stains, 
the bark extracts, formalin. Phenol Sodique, and ammonium hydroxide. 
For instance, if a solution of anhydrous sodium carbonate 1/200 G. M. 
is used, the molecular weight of sodium carbonate is found, which is 106.10. 
106.10 grams of salt added to a liter of water makes a gram molecular solu- 
tion, and a solution 1/200 G. M. means that i cc. of the G. M. solution is 
added to 199 cc. of water. 
The chemicals used were bought as chemically pure. 
But one substance was injected into a single tree. In a few cases, all 
of which are indicated in a following list, stronger solutions were used in the 
later than in the earlier injections in a tree. 
Number of Trees Injected 
Usually three or more trees were injected with the same substance. The 
exceptional cases in which fewer than three trees were injected are as 
follows: But one tree injected: methyl alcohol. Phenol Sodique, oil of bitter 
almonds, and para cresol. But two trees injected: zinc chloride, barium 
chloride, colloidal metallic silver, and colloidal metallic mercury. The 
largest number of trees injected with one salt was thirteen, injected with 
lithium carbonate solutions of different dilutions. Nineteen check trees 
were injected with water. 
Some of these trees were injected two years in succession, some three 
years, the greatest number but one year. 
The injections were made in 1912, 191 3, and 1914. In 1913 a record of 
the weather was kept together with a record of the daily intake of the trees, 
so that all remarks on the rate of intake of the trees will be confined to the 
