120 
OTIS F. CURTIS 
Between rings: Starch was very abundant in medullary rays and pith. 
Below lower ring: There were distinct traces of starch in the medullary 
rays and in a few pith cells. 
Lower part of upper ring appeared the same as between rings. 
Upper part of upper ring: Starch was fairly abundant but distinctly less 
abundant than in the lower part. 
Lower part of lower ring: Starch was fairly abundant in medullary rays 
and pith but less in the latter than between the rings. 
Upper part of lower ring: The starch content was the same as between the 
rings. 
Check: Traces of starch were present in smaller medullary rays and in 
the pith but less than just below the lower ring. This was true for all parts 
corresponding to those tested as described above. 
The stems were cut into pieces 12 cm. long, from which all tissues outside 
the cambium were removed. These pieces were centrifuged, and the sap 
was tested for invert sugar after hydrolyzing with hydrochloric acid. 
The exact amount of sap remaining after removing 4 cc. samples was not 
determined as the tubes were rinsed out with water. 
Table 16. Fagus grandiflora. Sugar obtained from centrifuged stems 
Green Weight 
of Sticks 
Mg. Invert 
Sugar in 4 Cc. 
Samples 
Mg. Invert 
Sugar in 
Remaining 
Sap 
Total Invert 
Sugar 
Obtained. 
Total Mg. 
Invert Sugar 
from Centri- 
fuged Sap 
per 100 Gms. 
of Xylem 
Check 
Above ring 
Between rings 
125.0 
1854 
219.6 
2.03 
1-25 
1.67 
1.08 
0.72 
3.II 
1.97 
543 
2.49 
1.06 
2.47 
The amounts of sap obtained were so small that the analyses cannot be 
fully depended upon, yet there seems to be clear indication that more sugar 
is present in the sap centrifuged from the xylem between the rings than 
from that above. 
Table 17. Fagus grandiflora. Sugar soluble in 80% alcohol hydrolyzed and expressed 
as invert sugar 
Mg. Invert Sugar in 
25 Cc. Samples 
Average 
Total Mg. Invert 
Sugar in the 50 
Gms. Dry Wood 
Check 
Above rings 
Between rings 
42.34 
42.30 
61.08 
43.7 
41.35 
60.20 
43.02 
41.825 
60.64 
344.16 
334.60 
485.12 
The wood was sawed into short sections about a millimeter in length. 
After drying in the oven, lots of fifty grams each of this material, including 
the sawdust, were extracted in 250 cc. of 80 percent alcohol at 37° for 24 
hours, and again in fresh alcohol for 72 hours. After heating to 80° the 
