268 MEASUREMENT OF THE CATALYTIC POWER OF CATALASE 
contains acetanilide enough to damage the enzym during the reaction 
(see below). We expect to repeat the experiments under standard condi- 
tions and ask the reader therefore to consider this paper as a preUminary 
account. 
The time in which the reaction on 2 cc. peroxide was completed was in 
one case: 
For 4 cc. extract, 15 mm. ( 15 X -g- sec); 
for 3 cc. extract, 21 mm. ( 21 X-8~sec.); 
for 2 cc. extract, 29.5 mm. ( 29.5 X -g" sec.) ; 
for I cc. extract, 59 mm. ( 59 X -g- sec.) ; 
for J cc. extract, 118 mm. (118 X-g-sec). 
We can state that the reaction time is inversely proportional to the 
amount of enzym, E (amount of enzym) X T (reaction time in units 
of -g" seconds) thus being constant. 
Again : 
For 4 cc. extract, E X T = 60; 
for 3 cc. extract, E X T = 63; 
for 2 cc. extract, E X T = 59; 
for I cc. extract, E X T = 59; 
for I cc. extract, E X T = 59. 
In another case we obtained these results: 
For 4 cc. extract, E X T = 24; 
for 3 cc. extract, E X T = 27; 
for 2 cc. extract, E X T = 26; 
for I cc. extract, E X T = 25; 
for J cc. extract, E X T = 24.5. 
These numbers strikingly show the value of the autographical method. 
Fig. 3. Autogram. Effects of different quantities of enzym. 
Figure 3 gives still another proof for 3 curves, taken with 4, 2, and I 
cc. of extract respectively, and 4 cc. lo-vol. H2O2. Different amounts of 
