Jan., 1921] 
WANN — FIXATION OF FREE NITROGEN 
21 
Tables 9, 9A, and 9B. Results of analyses of mineral nutrient agar cultures of green algae. 
Experiment of 1919, Growth period, 235 days. Nitrogen source^ calcium nitrate 
9. Medium without glucose 

No. 
Culture' 
Species 
Growth 
Total 
Mg. N 
Found 
Wt. of 
Medium, 
Grams 
iVJg, IN pCl 
100 Gr. 
Medium 
Average 
TV/T cr T rxcc 
or Gain, 
ICQ Gr. 
Gam 
Percent 

Check 
Ster. 
24.202 
147.90 
16.364 

Check 
Ster. 
24.146 
147-95 
16.320 
16.755 

Check 
Ster. 
26.045 
148.15 
17.580 
I 
Chlorella vulgaris 
+ + + 
25.822 
147-25 
17-536 1 
I 
Beyr, 
-1- 4- -1- 
26.939 
T /I *7 T e 
t8 ICt'7 
i. T '7 87/1 
1 / .0/4 
—1— T T T r» 
6. 
I 
+ + + 
26.199 
147.35 
17.780 1 
2 
Sticho coccus sp. 
+ 
22.735 
147-30 
15-435 
2 
+ + 
26.464 
147-45 
17.948 
> 17.058 
+ 0.303 
2 
+ 
26.241 
147-50 
17.791 J 
5 
Chlorella (?) sp. 
+ + 
27.322 
147.60 
I8.5II 1 
5 
+ + 
26.520 
147.90 
17-931 
\ 18.689 
+ 1-934 
II.5 
5 
+ + 
28.978 
147-65 
19.626 
6 
Scenedesmus (?) sp. 
+ + 
22.875 
147-85 
15-472 1 
6 
+ + 
24.341 
147.90 
16.458 
16.522 
-0.233 
6 
+ + 
26.073 
147-85 
17-635 
II 
Chlorella (?) sp. 
+ + + 
17. 121 
148.25 
11-549 1 
II 
+ + + 
17.749 
148.15 
11.980 
■ 11-439 
-5-316 
II 
+ + + 
16.046 
148.75 
10.787 J 
Average all cul- 
tures (omitting 
species no. 11) 
17-311 
+0.556 
9A. Same nitrogen source, medium with i percent glucose 
Check 
Ster. 
23-055 
148.70 
15.504 
Check 
Ster. 
22.553 
148.85 
15.152 
15.640 
Check 
Ster. 
24.298 
149.40 
16.264 
I 
Chlorella vulgaris 
+ + + + + 
34-535 
148.70 
23.225 ' 
I 
Beyr. 
+ + + + +* 
33.948 
148.50 
22.861 
■ 22.963 
+ 7.323 
47.- 
I 
+ + + + + 
33-864 
148.50 
22.804 
2 
Stichococciis sp. 
++++++ 
35-024 
148.50 
23.585 1 
2 
++++++ 
30.820 
148.50 
20.754 
> 22.440 
+6.800 
43.5 
2 
Chlorella (?) sp. 
++++++ 
34.186 
148.75 
22.982 ^ 
5 
+ + + + + 
34-849 
148.70 
23.436 ' 
5 
+ + + + + 
35-799 
148.90 
24.042 
^23.677 
+8.037 
51-4 
5 
+ + + + + 
35-071 
148.90 
23.553 j 
6 
Scenedesmus (?) sp. 
+ + + + + 
34-388 
148.75 
23.118 
6 
+ + + + + 
34-975 
149.15 
23.450 
' 23.434 
+ 7.794 
49.7 
6 
Chlorella (?) sp. 
+ + + + + 
35.338 
148.90 
23.733 
II 
++++++ 
31-714 
148.90 
21.299 
II 
++++++ 
32.831 
149.00 
22.034 
^21.319 
+5.679 
36.3 
II 
Protococcus sp. 
++++++ 
30.750 
149.10 
20.624 
7 
7 
+ 
+ 
24.243 
24.578 
149.05 
149-15 
16.265 1 
16.479 1 
> 16.372 
+0.732 
4.7 
3 
Protosiphon 
+ + + + 
25.360 
149-15 
17.003 1 
> 17 372 
+ 1.732 
II. 
3 
botryoides 
+ + + + 
26.477 
149.25 
17.740 J 
Average all cul- 
tures 
21.082 
+5.442 
34.8 
