Jan., i92i] WANN — FIXATION OF FREE NITROGEN I9 
7B. Same nitrogen source. Medium with i percent mannite 
— 
Check 
Ster. 
20.520 
148.55 
13.814 
— 
Check 
Ster. 
21.162 
148.80 
14.222 
' 13.993 
— 
Check 
Ster. 
13.872 
99.50 
13-942 , 
I 
Chlorella vulgaris 
+ + +* 
20.966 
148.35 
14-133 
I 
Beyr. 
+ + + 
22.070 
148.40 
14.872 
> 14.321 
+0.328 
I 
+ + + 
20.729 
148.50 
13-959, 
2 
Stichococcus sp. 
+ + 
20.938 
148.65 
14.085 
2 
+ + 
19-570 
148.70 
13. 161 
' 13.658 
-0.335 
2 
20.408 
148.65 
13.729 1 
5 
Chlorella (?) sp. 
+ + + 
2 1 .064 
148.50 
14.185 1 
5 
+ + + 
20.603 
148.70 
13.855 
> 13.780 
-0.213 
c 
0 
+ + + 
10.76s 
148.60 
6 
Scenedesmus (?) sp. 
+ + 
21.637 
148.80 
14.541 1 
6 
+ + 
20.087 
148.70 
13.508 
> 14.061 
+0.068 
c 
0 
1 1 
2 1 .008 
r aQ fir- 
140.05 
14.133 , 
II 
Chlorella (?) sp. 
+ + 
19.905 
148.80 
13.377 
II 
+ + • 
20.701 
148.90 
13.903 
13.584 
—0.409 
II 
+ + 
20.073 
149.00 
13.472 ^ 
7 
7 
Protococcus sp. 
+ 
+ 
20.226 
20.380 
149.00 
149.00 
13.575 1 
13.678 1 
■ 13.627 
-0.366 
3 
Protosiphon 
+ + + 
20.757 
149-15 
13.917 
> 14.040 
+0.047 
3 
botryoides 
+ + + 
21.134 
149.20 
14.162 , 
Average all cul- 
tures 
13.867 
—0.126 
Tables 8, 8 A, and 8B. Results of analyses of mineral nutrient agar cultures 
of green algae. Experiment of 1919. Growth period, 255 days. Nitrogen source, 
AMMONIUM NITRATE 
8. Medium without glucose or mannite 
No. 
Culture 
Species 
Growth 
Total Mg. 
N Found 
Wt. of 
Medium, 
Grams 
Mg. N per 
ICQ Gr. Average 
Medium 
Mg. Loss 
or Gain, 
100 Gr. 
Check 
Ster. 
26.704 
147.45 
18. Ill ^ 
Check 
Ster. 
26.789 
147.95 
18.107 
' 17-736 
Check 
Ster. 
25.189 
148.25 
16.991 
I 
Chlorella vulgaris 
+ + + + +* 
27.015 
147.65 
18.292 
I 
Beyr. 
+ + + 
27.029 
147.25 
18.356 
18.308 
+0.672 
I 
+ + + 
26.958 
147.50 
18.277 1 
2 
Stichococcus sp. 
+ +* 
26.576 
147.35 
18.036 1 
2 
+ 
26.166 
147.50 
17.740 ) 
17.989 
+ 0.253 
2 
+ 
26.888 
147.80 
18.192 ^ 
5 
Chlorella (?) sp. 
+ + + 
25.543 
147.65 
17.300 1 
5 
+ + 
25.413 
147.90 
17.183 
17.198 
-0.538 
5 
+ + 
25-274 
147.70 
17. 112 
6 
Scenedesmus (?) sp. 
+ + + 
26.619 
147.75 
18.016' 
6 
+ + + 
26.194 
147.95 
17.705 
• 17.866 
+ 0.130 
6 
+ + + 
26.421 
147.80 
17.876, 
II 
Chlorella (?) sp. 
+ + + 
20.248 
147.90 
13.690^ 
II 
+ + + 
20.615 
147.95 
13-934 
- 14.018 
-3-718 
II 
+ + + + 
21.380 
148.15 
14-431 
Average all cul- 
tures (omitting 
species no. 11) 
17.840 
+ 0.104 
