l6 AMERICAN JOURNAL OF BOTANY [Vol. 8 
Tables 4 and 4A. Results of analyses of mineral nutrient agar cultures of green algae. 
Experiment of IQ17-18. Growth period, zyo days. Nitrogen source, ammonium nitrate 
4. Medium without glucose 
Culture 
Growth 
Total 
Mg. IN 
Found 
Wt. of 
Medium, 
Grams 
Mg. N per 
loo Gr. 
Medium 
Average 
Mg. Loss 
or Gain, 
100 Gr. 
Gain 
Percent 
No. 
Species 
I 
I 
2 
2 
5 
5 
6 
6 
Check 
Check 
Check 
Chlorella vulgaris 
Beyr. 
Stichococcus sp. 
Chlorella (?) sp. 
Scenedesmus (?) sp. 
Con.* 
Con.* 
+ + +* 
+ + +* 
( + +) 
( + +) 
+ + + + 
+ + + 
+ + + 
23-569 
23-885 
22.445 
22.410 
23.674 
24.921 
23- 569 
25.729 
24- 377 
25- 940 
Lost 
146.90 
147-95 
148.25 
146.20 
146.25 
146.45 
146.70 
146.90 
147.40 
147.60 
147-75 
16.044 ' 
16.144 
15-175 
15-328 1 
16.188 J 
17.017 1 
16.066 1 
17-515 1 
16.538 J 
17-574 1 
i 
• 15-788 
> 15-758 
> 16.542 
< 17.027 
^ 17-574 
— 0.030 
+0.754 
+ 1.239 
+ 1.786 
4.7 
7.8 
II.3 
Average all cul- 
tures 
16.725 
^ 1 
+0.937 1 5-9 
4A. Same medium, with i percent glucose 
I 
Check 
Check 
Check 
Chlorella vulgaris 
Ster. 
Ster. 
Ster. 
( + + + +) 
24.323 
23.446 
24.095 
32.747 
147.75 
148.60 
145.90 
146.90 
15-794^ 
15.778 
16.525^ 
21.978 1 
16.032 
^22.136 
21.910 
■ 22.394 
> 22.179 
+6.104 
+5.878 
+6.362 
+6.147 
38. 
36. 
39.6 
38.3 
I 
2 
2 
5 
5 
6 
6 
Beyr. 
Stichococcus 
Chlorella (?) sp. 
Scenedesmus (?) sp. 
( + + +) + 
++++++ 
++++++ 
+ + + + + 
+ + + + + 
+ + + + + 
+ + + + + 
32.817 
32.269 
32.203 
33.291 
33.028 
33.624 
32.168 
147.20 
147.10 
147.50 
147.90 
148.25 
148.25 
148.40 
22.294 J 
21.987 1 
21.832 
22.509 1 
22.278 1 
22.681 1 
21.676 j 
Average all cul- 
cultures 
22.155 
+6.123 
38. 
Tables 5 and 5A. Results of analyses of mineral nutrient agar cultures of green algae. 
Experiment of iQiy-i8. Growth period, 2QY days. Nitrogen source, calcium nitrate 
5. Medium without glucose 
Culture 
Growth 
Total 
Mg. N 
Found 
Wt. of 
Medium, 
Grams 
Mg. N per 
100 Gr. 
Medium 
Average 
Mg. Loss 
or Gain, 
100 Gr. 
Gain 
Percent 
No. 
Species 
I 
I 
2 
2 
5 
5 
6 
6 
6 
Check 
Check (Loss) 
Chlorella vulgaris 
Beyr. 
Stichococcus sp. 
Chlorella (?) sp. 
Scenedesmus (?) sp. 
Ster. 
Ster. 
+ + + 
+ + + 
+ + 
+ + 
+ + + 
+ + + 
+ + + 
+ + + 
+ + + 
24.850 
21.182 
23.797 
23.586 
23.990 
23.902 
27.219 
28.079 
27.079 
24.078 
23.393 
147.35 
148.25 
145-65 
145.80 
146.05 
146.55 
147.30 
147.55 
147.80 
148.00 
148.25 
/ 16.903 \ 
114.289/ 
16.338 1 
16.177 j 
16.425 1 
16.310 j 
18.479 ^ 
19.030 1 
18.322 
16.269 
15.105 J 
ti6.903 
■ 16.258 
■ 16.368 
■ 18.755 
16.565 
-0.645 
-0.535 
+ 1.852 
-0.338 
II. 
Average all cul- 
tures 
16.987 
+0.084 
