14 
AMERICAN JOURNAL OF BOTANY 
[Vol. 8 
Tables i and iA. Results of analyses of mineral nutrient agar cultures of green algae. 
Experiment of igiy-igiS. Growth period, 2jo days. Nitrogen source, glycocoll 
I . Medium without glucose 
No. 
Culture 
Species 
Growth 
Total Mg. 
N Found 
Wt. of 
Medium, 
Grams 
Mg. N per 
100 Gr. Average 
Medium 
Mg, Loss 
or Gain, 
100 Gr. 
I 
2 
2 
5 
5 
6 
6 
Check 
Check 
Check 
Chlorella vulgaris Beyr. 
Stichococcus sp. 
• 
Chlorella (?) sp. 
Scenedesmus (?) sp. 
Con.* 
Ster. 
Ster. 
+ + + t 
+ + + 
+ + + +* 
+ + + 
+ + + 
+ + +* 
+ + + 
+ + + 
32.655 
32.389 
32.796 
31.823 
32.036 
31.859 
32.230 
31.965 
32.478 
32.354 
32.053 
145.90 
147.10 
147.45 
144.90 
145.15 
145.30 
145.75 
146.15 
146.40 
146.70 
147.00 
22.382 
22.018 
22.242 
21.962 ' 
22.071 ^ 
22.926 ' 
22.113 , 
21.871 
22.184 
22.055 
21.805 J 
> 22.214 
> 22.017 
= 22.020 
> 22.028 
21.930 
-0.197 
-0.194 
-0.186 
— 0.284 
Average all cultures 
21.999 
-0.215 
lA. Same medium, with i percent glucose 
I 
I 
2 
2 
5 
5 
6 
6 
Check 
Check 
Check 
Chlorella vulgaris Beyr. 
Stichococcus sp. 
Chlorella (?) sp. 
Scenedesmus (?) sp. 
Ster. 
Ster. 
Ster. 
+ + + + + 
+ + + + + 
+ + + + + 
+ + + + + 
+ + + + + 
+ + + + + 
+ + + + + 
+ + + + + 
31.646 
31.841 
31.735 
31.010 
31.859 
31.438 
31.452 
30.656 
31.328 
31.540 
3I.6II 
146.70 
148.00 
148.15 
146.55 
146.60 
146.55 
146.65 
147.20 
147.20 
147.50 
147.90 
21.572 
21.514 
21.421 
21.160 ) 
21.732 \ 
21.452 1 
21.447 1 
20.826 1 
21.283 i 
21.383 \ 
21.373 j 
21.502 
21.466 
^21.450 
> 21.055 
21.378 
-0.056 
-0,052 
-0.447 
— 0.124 
Average all cultures 
1 21.332 
— 0.170 
Tables 2 and 2A. Results of analyses of mineral nutrient agar cultures of green algae. 
Experiment of igiy-igiS. Growth period, 234 days. Nitrogen source, asparagine 
2, Medium without glucose 
Culture 
Growth 
Total Mg. Wf of 
N Found Medium, 
j Grams 
Mg. N per 
100 Gr. Average 
Medium 
Mg. Lo- s 
or Gain, 
100 Gr. 
No. 
Species 
I 
I 
2 
2 
5 
5 
6 
6 
Check 
Check 
Check 
Chlorella vulgaris Beyr. 
Stichococcus sp. 
Chlorella (?) sp, 
Scenedesmus (?) sp. 
Ster, 
Ster. 
Ster. 
+ + 
+ + 
+ + 
+ + 
+ + + 
+ + + 
+ + + 
+ + + 
27.808 
28.162 
27.967 
27.861 
27.614 
28.410 
27.755 
27.667 
27.454 
27,614 
27.578 
146.50 
147.25 
147.30 
146.20 
146.30 
146.30 
146.35 
146.50 
146.60 
146.90 
146.95 
18.982 
19.125 
18.987 . 
19.057 1 
18.875 J 
19.419 1 
18.965 J 
18,885 1 
18.727 J 
18,798 1 
18,767 J 
J9.O3I 
18.966 
\ 19.192 
> 18,806 
> 18.783 
-0.065 
+O.161 
-0.225 
— 0.248 
Average all cultures 
1 
18,937 
— 0.094 
* Checks or cultures which were contaminated are designated with an asterisk. 
t Relative growth of the cultures is indicated by plus signs, as follows: + = "slight," 
+ + = "fair," + + + = "very fair," + + + + = "good," + + + + + = "very good," 
+ + + + + + = "luxuriant," 
