AMERICAN JOURNAL OF BOTANY 
[Vol. 8 
Table 2. Partial volume-molecular concentration of the salts employed in the cultures of the 
series in group 2 
Culture No. 
Series 4 (2.1 Atm.) 
' Series 5 (4.2 Atm.) 
KH._,P04 
NaNOg 
MgS04 
KH2PO4 
NaNO.3 
MgS04 
RiCi 
.00444 
.00414 
•O4157 
.00888 
.00860 
.08648 
R1C2 
.00444 
.00829 
•03637 
.00888 
.01721 
.07567 
RiC^ 
.00444 
.01243 
.03118 
.00888 
.02581 
.06486 
R1C4 
.00444 
.01658 
.02598 
.00888 
.03442 
•05405 
RiC=; 
.00444 
.02072 
.02078 
.00888 
.04302 
.04324 
R1C6 
.00444 
.02487 
•01559 
.00888 
.05162 
•03243 
R1C7 
.00444 
.02901 
.01039 
.00888 
.06023 
.02162 
R1C8 
.00444 
.03316 
.00502 
.00888 
.06883 
.01081 
R2C1 
.00888 
.00414 
•03637 
.01776 
.00860 
.07567 
R2C2 
.00888 
.00829 
.03118 
.01776 
.01721 
.06486 
R2C^ 
.00888 
.01243 
.02598 
.01776 
.02581 
•05405 
R2C4 
.00888 
.01658 
.02078 
.01776 
.03442 
.04324 
R2C5 
.00888 
.02072 
•01559 
.01776 
.04302 
•03243 
R2C6 
.00888 
.02487 
.01039 
.01776 
.05162 
.02162 
R2C7 
.00888 
.02901 
.00502 
.01776 
.06023 
.01081 
R^Ci 
.01332 
.00414 
.03118 
.02664 
.00860 
.06486 
R^C2 
.01332 
.00829 
.02598 
.02664 
.01721 
•05405 
R^C^ 
.01332 
.01243 
.02078 
.02664 
.02581 
.04324 
R^CzL 
.01332 
.01658 
•01559 
.02664 
.03442 
•03243 
R^C=; 
.01332 
.02072 
.01039 
.02664 
.04302 
.02162 
R^C6 
.01332 
.02487 
.00502 
.02664 
.05162 
.01081 
R4C1 
.01776 
.00414 
•02598 
•03552 
.00860 
•05405 
R4C2 
.01776 
.00829 
.02078 
•03552 
.01721 
.04324 
R4C3 
.01776 
.01243 
•01559 
•03552 
.02581 
.03243 
R4C4 
.GI776 
.01658 
•01039 
•03552 
.03442 
.02162 
R4C5 
.01776 
.02072 
.00502 
•03552 
.04302 
.oio8f 
R5C1 
.02220 
.00414 
.02078 
.04440 
.00860 
.04324 
R5C2 
,02220 
.00829 
•01559 
.04440 
.01721 
.03243 
R5C3 
.02220 
.01243 
•01039 
.04440 
.02581 
.02162 
R5C4 
.02220 
.01658 
.00502 
.04440 
.03442 
.01081 
R6C1 
.02664 
.00414 
•01559 
.05328 
.00860 
.03243 
R6C2 
.02664 
.00829 
■01039 
.05328 
.01721 
.02162 
R6C3 
.02664 
.01243 
.00502 
•05328 
.02581 
.01081 
R7C1 
.03108 
.00414 
•01039 
.06216 
.00860 
.02162 
R7C2 
.03108 
.00829 
.00502 
.06216 
.01721 
.01081 
R8C1 
•03552 
.00414 
.00502 
.07104 
.00860 
.01081 
For each series a stock solution of each of the salts was made up with 
distilled water to such a concentration that I cc. of the stock in 50 cc. of 
the culture solution produced in any culture one tenth of its total required 
salt concentration. Thus, the culture in which the three salts KH2PO4, 
Ca(N03)2, and MgS04 produced, respectively, i/io, 6/10, and 3/10 of the 
total concentration, received of these stocks i cc, 6 cc, and 3 cc, in the order 
given. Each culture, therefore, received a total of 10 cc. of stock solutions, 
leaving 40 cc. to be supplied by other means. The sugar stock was made 
up to 5/4 the concentration desired in the finished culture; thus, 40 cc of 
this stock contained the proper amount of sugar for the 50 cc culture. 
Ferrous sulphate equivalent to o.oi gram FeS04 per liter of finished nutrient 
was added directly to the sugar stock. 
The stock solutions were weighed and sterilized separately at 100° C. 
for one hour on three consecutive days. The loss by evaporation was 
