62 Transactions of tJie Boyal Society of South Africa. 
A year later, in December, 1913, I found in the cloaca of a Chamaeleon 
pumihts,''- also on Eobben Island, numerous flagellate parasites belonging 
to the same genus, and possibly of an identical or allied species. 
Several smears were taken, fixed for ten seconds in Osmic acid fumes, 
then thirty minutes in absolute alcohol and stained according to Giemsa. 
These specimens appear to be broader, blunter, and somewhat shorter 
than the HerjMtomonas found in Scatopliaga liottentota. The tropho- 
nucleus stains a clear, light purple hue, the kinetonucleus is sharply defined 
as a compact, short rod, generally placed transversely to the length of the 
protozoon. Its position is generally half-way between trophonucleus and 
the flagellar end. In some instances, especially in pear-shaped stumpy 
flagellate forms, the kinetonucleus has moved up so as to separate from 
the trophonucleus only by a space not superior to 3 ^x. In single speci- 
mens the kinetonucleus appears to be slightly curved with its concavity 
facing the flagellum and a faint, vacuole-like clear space can be detected 
between this structure and the basal granule of the flagellum. Some of 
the flagella appear, moreover, to be covered by a sheath or slight membrane 
which encloses them to the point where they enter the cytoplasm. 
Dividing and biflagellar forms are not rare in the specimens examined. 
Multiplication takes place by binary fission of the long flagellate stages. 
When the process of fission is very active, the resulting flagellates are 
long and very thin, so that in an unfixed specimen they dart about in the 
fluid like spirilla ; the nucleus appears also to be reduced in size and 
is hardly distinguishable. 
Development of non-flagellate rounded forms appears to take place by 
the production of a thin, short flagellum, which increases in length and 
thickness, whilst the body of the protozoon gets longer and thinner, the 
kinetonucleus moving towards the flagellar end in the process, till it 
reaches its usual midway position. 
Though in single instances rounded forms were noticed which ap- 
peared to be devoid of a blepharoplast, still no LeisJwiania-\ike stages 
could be detected in the cells of the epithelial lining of the cloaca. 
Smears from the liver and spleen also gave a negative result from this 
point of view. 
Measurements : Length of flagellate bodies, 25 to 75yu; breadth, 2 to 
10 fji ; diameter of trophonucleus, 2*5 to 4 ju. 
Though slight differences in size and appearance are noticeable in 
these Hcrpetomonads from different sources, still they certainly are not 
more marked than those found in samples taken from the same artificial 
culture of Leislimania at a few days' interval, therefore it does not seem 
advisable at the present stage of our knowledge to postulate two distinct 
species of protozoa. It does not seem excluded that a chamaeleon can 
* Kindly identified by Dr. Peringuey, of the South African Museum, Capetown. 
