Deterioration of Maize Infected with Diplodia zeae. 
237 
Method. — Five grams of the substance was extracted overnight with 
15 CO. of a 50 per cent, aqueous solution of Glycerin. The extract was 
then filtered through paper ; 1 c.c. placed in a test tube and 4 to 5 drops 
of a 3 per cent, solution Hydrogen Peroxide added. 
B. — Chemistry of Maize Inoculated with the Fungus in the 
Garden, Compared with the Chemistry of Healthy Control 
Maize. 
In view of the fact that Maize may undergo certain changes when 
sterilized in an autoclave, it was thought desirable to carry out the tests 
already mentioned, also on maize inoculated in the garden, and to com- 
pare the results with that obtained from healthy maize which had stood 
alongside the inoculated. 
Maize cobs (Fig. 2) inoculated on the 16th of March, 1914, by 
puncturing through the husks with a sterilized needle dipped into 
water containing the spores of the fungus, were picked on the 5th of 
May, 1914, and placed in the sun to dry. Control cobs were picked 
at the same time and treated similarly. 
On the 12th of May, 1914, the kernels of the diseased and control 
cobs respectively were ground up separately in a coffee mill, the control 
being ground first. 
With the ground-up material the following tests were made, following 
the methods described in part A of this paper. 
BesuU. — 
Control 
Inoculated. 
Very small bubbles after a little while. 
The bubbles increase in size and the 
effervescence becomes more rapid. 
No bubbles. 
(1) Acidity. 
Control . . . 
Inoculated 
(2) Moisture and Ash. 
Percentage Percentage 
Moisture. Ash. 
Control 
Inoculated 
5-5 1-48 
7-5 1-94 
