344 
Transactions of the Royal Society of South Africa. 
It was thus possible to obtain bacillary emulsions which, with suitable 
complements, showed little anti-complementary effect in the quantities used. 
Bacillary extracts have been extensively used in place of simple emul- 
sions. These were originally employed by Wassermann and Bruck, and 
various workers have preferred them on the ground that they are less anti- 
complementary than emulsions. I have prepared extracts by Dean's method 
of alternately freezing and thawing emulsions and then removing the 
Table XXI.~X?/sis of 0-5 
c.c. 5 per cent. Suspension Ox Blood + 5 Doses 
Immune Body. 
Antiserum 
to Strain 1, 
A I {B. No. 
71 McC). 
0-01 C.C. 
0-005 „ 
0-001 „ 
0-0005 „ 
0-0001 „ 
0-01 
0-005 „ 
0-001 „ 
0-01 „ 
0005 „ 
0001 „ 
0-01 
O-005 
0-001 
0-01 
0-005 
0-001 
Bacillary 
emulsion 
O'i c.c. 
Strain 
1, Al 
(B. No. 
71) 
I Strain \ 
J \ 
\ Strain / 
[_], A6 (5. 1 
j neapoli- "j 
/ tanus) [ 
] Strain 1, 
A2 (B. 
1 co/i com- 
j munis) 
] Strain 1, ( 
\ A4(/?. \ 
1 Griinthal) \ 
Doses of complement. 
2 D. 
i D. 
7 D. 
12 D. 
20 D. 
0 
0 
0 
0 
0 
0 
0 
0 
0 
0 
0 
0 
0 
0 
0 
0 
0 
0 
0 
0 
0 
Dist. 
Just c. 
0 
0 
0 
0 
0 
0 
0 
0 
Trace 
Com. 
0 
Dist. 
Mkd. 
Al. com. 
Com. 
0 
0 
0 
0 
Trace 
0 
0 
0 
Trace 
V. mkd. 
0 
Mkd. 
Mkd. 
V. mkd. 
Com. 
0 
0 
0 
0 
Trace 
0 
0 
0 
Trace 
Mkd. 
0 
Trace 
Mkd. 
V. mkd. 
Com. 
0 
0 
Dist. 
Mkd. 
Al. com. 
0 
Dist. 
Mkd. 
V. mkd. 
Al. com. 
Dist. 
V. mkd. 
Al. com. 
Com. 
Com. 
Complement 
deviated by- 
emulsion 
alone. 
Mkd. 
Mkd. 
V. mkd. 
Mkd. 
Com. 
Just c. 
Just c. 
Just c. 
Just c 
Com. 
0'025 c.c. antiserum alone deviated 2 D. of complement. 
O'Ol „ „ „ „ no complement. 
bacteria by centrifugalisation. These extracts were found no less inhibitory 
in certain amounts with various specimens of complement than the ordinary 
emulsions. Throughout the experiments emulsions have been used as 
antigen ; they are easily prepared, and with suitable complements exhibit 
little inhibitory effect. 
The immune sera used were those already referred to in connection with 
the agglutination experiments. 
Gruinea pig's serum was used as complement, and ox-blood corpuscles 
sensitised with five doses of a haemolytic immune body (from the rabbit) 
was employed as the haemolytic system. 
