A Study of the B. coli Group, 
345 
The method of carrying out the tests, where careful comparisons were 
made of the deviation of an antiserum along with different bacillarj strains, 
was as follows : varying quantities of the serum were added to a fixed 
quantity of the antigen, and then a quantitative estimation of the amount of 
complement deviated by these mixtures was made by adding varying amounts 
of complement from 8 M.H.D up to 20 M.H.D. (for 0-5 c.c. of the test 
blood suspension), incubating the mixtures for 1^ hours at 37° C, and then 
Table XXIT. — Lysis of 0*5 ex. 5 per cent. Suspension Ox Blood + 5 Boses 
Immune Body. 
Antiserum 
to Strain 1, 
A3 {B. vesi- 
cidosus). 
Bacillary 
emulsion 
0-4 c.c. 
Doses of complement. 
Complement deviated 
by emulsion alone. 
1 .1.. 
5 D. 
t 
10 D. 
15 D. 
20 D. 
2 D. 
5 D. 
7 D. 
0025 c.c. 
j 0 
0 
0 
0 
0 
001 „ 
1 Strain | 
0 
0 
0 
0 
0 
0005 „ 
1 1, A3 j 
! 0 
0 
0 
0 
0 
V. mk. 
Al. com. 
Com. 
0001 
r {B. vesi- \ 
0 
0 
0 
0 
0 
0 0005 „ 
1 cidosiis) 1 
0 
0 
Mkd. 
Al. com. 
Com. 
00001 „ 
Dist. 
Mkd. 
Com. 
0-025 „ 
0 
0 
0 
0 
Dist. 
0-01 „ 
] Strain j 
0 
0 
Dist. 
V. mkd. 
Com. 
Dist. 
Al. com. 
Com. 
0-005 „ 
1 2, A3 1 
0 
Trace 
Mkd. 
Com. 
Com. 
0-001 „ 
Trace 
Mkd. 
Com. 
Com. 
Com. 
0-025 „ 
) Strain [ 
0 
0 
0 
F. tr. 
Dist. 
0-01 
1 1, A4 (B.J 
0 
0 
Dist. 
Al, com. 
Com. 
Dist. 
Al. com. 
Com. 
0-005 „ 
i Oriln- 1 
0 
Trace 
V. mkd. 
Com. 
Com. 
0-001 „ 
) thai) [ 
Trace 
Dist. 
Com. 
Com. 
Com. 
0-025 „ 
\ Strain 1, / 
! A9 (B. 1 
0 
0 
0 
F. tr. 
Mkd. 
001 „ 
0 
0 
Trace 
V. mkd. 
Com. 
Dist. 
Al. com. 
Com. 
0005 „ 
[. No. 106 -j 
0 
0 
Dist. 
Com. 
Com. 
0 001 „ ' 
I 
1 
McCon- 
j key) ( 
Trace 
Dist. 
Com. 
Com. 
Com. 
0-025 c.c. antiseriim alone deviated 1 D. of complement. 
0-01 „ „ „ „ no complement. 
adding 0 5 c.c. of the blood suspension. After a further hour's incubation 
the results were read. 
Control tests were also carried out to determine the number of doses of 
complement absorbed by the antigen and immune serum respectively. At 
the same time the dose of complement after incubation for 1|- hours was 
ascertained by adding suitable amounts of the complement-serum to tubes 
containing a volume of salt solution equal to that of the antigen used in the 
tests, incubating along with tlie other tubes and then adding the test 
corpuscles. 
The number of doses of complement deviated was taken as one less than 
