292 
WILLIAM H. BROWN 
medium chrom-acetic or Flemming's solution and stained with Heiden- 
hain's haematoxylin or Flemming's triple stain. 
For comparison with the variety inigneum the form of Pyronema 
confluens (omphalodes) described by Seaver ('09), which was grown 
as previously described, was prepared in the same way as the variety. 
In the figures of his strain, which in this discussion will be called the 
normal form, Seaver has figured trichogynes fused to the antheridia. 
Owing to the many excellent dcvscriptions and figures of Pyronema 
confluens, particularly the recent ones of Harper ('00) and Claussen 
('12), it seems unnecessary here to more than point out the differences 
between the variety inigneum and the previous descriptions. The 
cytological features observed are very similar to those described by 
the writer (Brown, '11) for Lachnea scutellata (L.) Sacc. and present 
no new features. 
The ascogonia of the variety inigneum are usually produced in 
rosettes of from fifteen to twenty. This is about twice the number in 
the normal form. De Bary ('84, p. 225), says that there are several, 
;at least two or three, ascogonia in a rosette, while Harper ('00) figures 
three. 
Many if not all of the ascogonia of a rosette of the variety inigneum 
are formed from the same hypha. The ascogonia are large, oval cells. 
Each is supported by a stalk, consisting of several cells which are 
smaller than the ascogonia, but larger than the other vegetative cells. 
The ascogonia and vegetative cells are all multinucleate. A long, 
slender, single-celled trichogyne is produced on the upper surface 
•of each ascogonium. This trichogyne was found to differ from the 
form described by Harper, and from the normal form in lacking a beak 
or snout-like projection at its apex. 
The antheridia are large, club-shaped, multinucleate cells and are 
quite similar to those figured by Harper ('00). The antheridia like 
the ascogonia are borne on stalks. In all cases in which the origin 
of an antheridium could be determined, it was found to be connected 
with the cell just below an ascogonium. 
In the normal form, the trichogynes and antheridia become con- 
nected in the manner that has been described by Harper ('00), while 
it is at this point that the variety inigneum differs markedly from 
Harper's description. In the variety inigneum, the antheridia and 
ascogonia are much more independent of each other than in the normal 
form. Frequently an ascogonium and the antheridium connected 
