DEVELOPMENT OF PYRONEMA CONFLUENS VAR. INIGNEUM 297 
knows, divisions have not been seen in the ascogonia of any of the 
Pezizineae in which a fusion of nuclei has been described at this point. 
Summary 
Pyronema confluens Tul. var. inigneum Brown agrees with the 
taxonomic description given by Saccardo for Pyronema confluens but 
differs from the ordinary form in that there is no fusion of antheridium 
and trichogyne. 
The cultural conditions for the growth of the variety also appear 
to differ from those for the normal form in that sterilization of the 
substratum is unnecessary. 
No fusion of nuclei was observed in the ascogonium or ascogenous 
hyphae. The only one observed occurred in the asci. 
There are apparently five chromosomes in all of the nuclear 
divisions. 
The work here reported was carried on at The Johns Hopkins 
University. The writer is indebted to Prof. D. S. Johnson for valuable 
suggestions and criticisms. 
Bureau of Science, 
Manila, P. I. 
