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T. H. GOODSPEED AND R. E. CLAUSEN 
the flowers on the plants the vegetative growth of which has been 
restricted by too large applications of nitrate are smaller than the 
flowers of the plants that have been treated with smaller amounts. 
IV. Discussion of Results 
With reference to the question of the stability of the character 
complex, corolla size, under conditions attending development, the 
results of measurements of flowers on plants of Nicotiana as tabulated 
and discussed above seem pertinent. East (loc. cit., p. 178) illustrated 
his conception of the expression "conditions attending development" 
by mentioning the behavior of vegetative and floral characters in 
Nicotiana under favorable and unfavorable conditions of greenhouse 
culture, under poor field conditions and under field conditions at their 
best, and the case of cuttings versus the plants in the field from which 
they came. As a result of his observations, unsupported by the 
presentation of numerical data. East states, first, that tobacco plants 
growing on land where all conditions are at their best for maximum 
vegetative growth bear flowers of the same size as those borne by 
sister plants which are forced to come to maturity on land not so 
favorable for maximum vegetative growth. Similar observations in 
the case of our cultures do not confirm any such conclusion. Very 
large, vigorous plants growing in rather rich garden soil bear distinctly 
larger flowers than sister plants not so large or vigorous growing in 
poor soil. For example the flowers of one plant of N. sylvestris chosen 
at random from the more vigorous group averaged 43.12 mm. in 
spread and 84.12 mm. in length of corolla as compared with 37.40 mm. 
and 78.57 mm. for the average spread and length of the most vigorous 
plant among the group growing on the poorer soil. The largest flower 
to be found on the 50 plants of this latter group gave a spread of 42 
mm. while over 20 flowers with a spread above 48 mm. were measured 
on only 10 plants of the former group. 
In East's cultures, secondly,* the flowers borne on plants in the field 
and on cuttings of these plants grown in small pots in the greenhouse 
are of the same size. The small amount of data available seems to 
show that rather a different condition of affairs is present in our 
cultures. Cuttings of a hybrid grown in pots in the greenhouse bore 
flowers the spread of which averaged nearly 4 mm. smaller than the 
spread of flowers on the plants in the field and over i mm. longer as to 
length of corolla. Cuttings of another Fi hybrid, Fi H38, received 
