542 
ADRIAN J. PIETERS 
(5) peptone o.i percent; (6) peptone 0.05 percent; (7j peptone o.oi 
percent; (8) peptone o.i percent, salts o.i percent; (9) peptone o.i 
percent, salts 0.05 percent; (10) peptone 0.1 percent, sucrose i per- 
cent; (11) peptone 0.1 percent, sucrose 0.5 percent. 
At the end of seven days a mat had been formed on the surface 
of the solutions, numbered i, 8 and 9 while the quantity of mycelium 
was in the following order, numbers i, 8, 9, 2, 3, 4, 10, 11, 5, 6, and 7. 
As before stated, no weighings were made for this species, but 
careful notes were taken of the apparent mass and condition. From 
these it appears that the complete solution, such as was suggested by 
Obel, solution number i, produced the best growth; that a mat was 
formed only in those solutions containing salts and that those solu- 
tions were much better for growth than those containing the same 
amount of peptone and sugar, but no salts. There was very little 
difference between the mass of mycelium produced by solutions of 
0.5 percent or i percent peptone or by peptone and sucrose, but the 
mass of mycelium out of 2 percent peptone was many times greater 
Table III 
A My a racemosa. Mycelia Grown Seven Days in Media Given in Column 2. Trans- 
ferred to Water at 18° 
3 
4 
s 
6 
7 
Sporangia, 
I Day. 
Sporan- 
gia. 2 
Days. 
Oogo- 
Da'y. 
Oogonia, 
2 Days. 
Oogonia, 5 Days. 
I 
2 
3 
4 
5 
6 
7 
8 
9 
10 
II 
Peptone 0.1%, sucrose 1%, 
salts 0.1% 
Peptone 2% 
Peptone 1% 
Peptone 0.5% 
Peptone 0.1% 
Peptone 0.05% 
Peptone 0.01% 
Peptone 0.1% + salts 0.1% 
Peptone 0. i % + salts 0.05 % 
Peptone 0.1% + sucrose 1% 
Peptone 0.1% 
+ sucrose 0.5%. ........ 
II-III 
II 
II-III 
II 
o-I 
0 
Very few 
II-III 
11 + 
0 
0 
IP 
II 
I 
III 
0 
0 
0 
o-I 
I 
0 
0 
o-I 
O-I 
0- I 
I 
1- II 
II 
II 
I 
0 
I 
II 
I 
o-I 
I 
I 
II 
II-III 
III 
III 
I 
II 
II 
P 
O-I 
II 
II 
III 
III 
III oogonia 
mostly empty 
III 
I 
III 
III oogonia 
mostly empty 
4 The number of sporangia given for the second day is in addition to those first 
recorded. 
^ The number of oogonia recorded for each day represents the number then 
present and not a number in addition to those previously recorded. 
