544 
ADRIAN J. PIETERS 
table shows that there was no agreement between mass of myceHum 
and number of oogonia, which fact may, however, have been due to 
the exhaustion of the myceHum by reason of the sporangia produced. 
By keeping the myceha after transfer to water at a temperature 
of 24°-25° C. the production of sporangia could be almost wholly 
prevented. This was done and the result is given in Table IV. The 
cultures used in this case were duplicates of those used for the previous 
experiment. 
It will be noted that at this temperature the production of sporangia 
was almost inhibited. The number of oogonia present at the end of 
five days was in every case great; but it was also noted that the 
oogonia on mycelium from i percent and from o.i percent peptone 
were large, though containing but one oospore, while in all other 
cases, except the mycelium from 2 percent peptone there were many 
empty oogonia, sometimes as many as 50 percent. In this series 
the largest number of oogonia, containing oospores, was produced 
by the mycelium having the smallest mass. 
From the same culture series another set, now seventeen days 
old, was taken and placed in water. After five days the number of 
oogonia found was as shown in Table V. 
Table V 
Achlya racemosa. Mycelia Grown Seventeen Days in Media Under Column 2. Trans- 
ferred to Water at 
I 2 3 
Media Oogonia in s^Days 
3 Peptone 1% Ill, oospores 2-3 in an oogonium. 
4 Peptone 0.5% Ill, large, 2-4 oospores. 
5 Peptone 0.1% 1 1-1 1 1 large oospores. 
6 Peptone 0.05% II, smaller than in o.i and few oospores. 
7 Peptone 0.01% I-II, few with oospores. 
8 Peptone 0.1%, salts 0.1% II-III, large and good. There have also 
been many sporangia. 
9 Peptone 0.1%, salts 0.05% o-I, there have been many sporangia. 
10 Peptone 0.1%, sucrose 1% II, small, but with oospores. 
11 Peptone 0.1%, sucrose 0.5% II, mostly with oospores. 
The table shows that, while above a certain concentration there 
is no relation between mass of mycelium and the number of oogonia 
produced, there is a concentration below which the lack of food is 
manifested in the smaller number of oogonia formed. In the case of 
