56o 
ADRIAN J, PIETERS 
on mycelium grown in the presence of levulose is especially interesting, 
since when this sugar is added to haemoglobin the resulting growth 
produces oogonia nearly all of which are accompanied by antheridia, 
while when dextrose is substituted for levulose the oogonia are larger 
but only a few of them have antheridia. 
In this series mycelium from each culture solution was trans- 
ferred to four dishes of haemoglobin and especial care was taken to 
have these transferred masses of as nearly the same size as possible, 
as it was intended to weigh the mycelium from the haemoglobin 
after the final oogonium record. This was done to see whether there 
was any relation between the number of oogonia and mass of my- 
celium produced in the haemoglobin. The column at the right in 
Table XV shows the total weight of the mycelium in the four dishes. 
It is true that slight differences in the masses originally placed in the 
haemoglobin probably did exist but when it is remembered that the 
figures represent the total weight from four dishes after several days 
growth, it will be seen that the small piece of mycelium originally put 
into the haemoglobin could in no case have exceeded one milligram in 
weight. The difference in weight between any two such masses there- 
fore would be represented at most by one unit in the fourth decimal 
place and could have no influence on the general result. Here again 
the evidence confirms the conclusion previously reached that there is 
no relation between the weight of mycelium and the number of 
oogonia produced by it. On March first another series was set up 
with S. monoica to confirm the results obtained by series VIII. Pep- 
tone and sugars only were used and the resulting data are presented 
in Table XVI. The cultures in this series were made in Joseph Kav- 
alier Bohemian glass flasks. 
Table XVI 
Series IX. S. monoica. Mycelia Out of Solutions Under Column 2. Transferred 
to 0.05 Percent Haemoglobin After Five Days. Weighed After Thirty Days 
in 200 cc. Solution. Weight in Milligrams. Number of Oogonia in 
25 Square Millimeters. Sugars All 1/50 Molecular 
Weight in 
Milligrams 
Sporangia 
3 Days 
Oogonia 
Peptone 1% 
" 0.1% 
" , 0.1% + dextrose. 
0.1% + levulose. 
" 0.1% -(- maltose. , 
" 0.1% + sucrose. . 
0244 
0270 
0196 
0773 
02675 
A few 
A few 
A few 
A few 
A few 
52 
85 
57 
JL5^ 
