564 
ADRIAN J. PIETERS 
Table XVIII 
Achlya prolifera. Mycelia Out of Pea and Peptone-Sucrose-Salts Solution into Solu- 
tions Under Column 2. Estimated Number of Oogonia and Oospores. 
The Figures for Oospores at Temperatures Given are Estimated 
Percentages 
Peptone-Sucrose-Salts, 
Pea Extract, 
Pea Extract, 
I 
2 
20°-22° C. 
IO°-l 
5°C. 
20°-22 
°c. 
i60-iJ 
3 
4 
5 
6 
7 
8 
9 
10 
No 
Media. 
Oog. 
Oosp. 
Oog. 
Oosp. 
Oog. 
Oosp. 
Oog 
Oosp. 
I 
Haemoglobin 0.1%. 
Few 
0 
O-I 
2 
0.05% 
I-II 
0 
II 
0 
3 
Peptone 0.01%. . . . 
O-I 
Few 
I 
Few 
4 
Peptone 0.01% 
+ dextrose M/500 
I-II 
II-III 
50 
Few 
5 
Peptone 0.01% 
+ levulose M/500 
O-I 
I-II 
6 
Peptone 0.01% 
+ maltose M/500 
II-III 
50 
III 
50 
7 
Peptone 0.01% 
+ sucrose M/200 
II-III 
50 
III 
90 
8 
Peptone 0.02%. . . . 
Growth only 
Great growth 
only 
9 
Peptone 0.02% 
+ dextrose M/iooo 
I-II 
25 
Few 
I 
0 
10 
Peptone 0.02% 
+ levulose M/250 
I 
II 
Peptone 0.02% 
+ sucrose M/300 
III 
95 
II 
25 
12 
Peptone 0.02% 
+ sucrose M/500 
Great growth 
only 
Great growth 
only 
13 
Peptone 0.03% 
+ dextrose M/300 
I 
50 
I-II 
Few 
14 
Peptone 0.03% 
+ maltose M/300 
II-III 
60 
O-I 
0 
15 
Peptone 0.03% 
+ sucrose M/300 
III 
95 
II 
50 
The experiment with 0.02 percent peptone and sucrose, number 
II, was among the earHer ones and it seemed reasonable to conclude 
that this was a favorable medium for the production of oogonia and 
that experiments at room temperature would give reliable results. 
Series V was therefore planned and carried through, the mycelia being 
grown in the culture media for seven days and then transferred, after 
being twice washed, to a solution containing 0.02 percent peptone 
and 1/300 molecular sucrose. The entire experiment was carried 
through at room temperature. The results are presented in Table 
XIX. In all cases more or less growth took place in the peptone- 
