328 Transactions of the Boyal Society of South Africa. 
hatched out on April 26, 1910. The larvae were placed on Heifer 1018 
on the 7th day of the fever reaction (May 30, 1910), and were collected 
during the three days previous to death, which occurred from East Coast 
Fever on June 7, 1910 ; during the time the ticks were feeding on 
Heifer 1013 the parasites of East Coast Fever were frequently met with, 
and all stages of development were found in the lymphatic glands. 
The larvae moulted into nymphee on August 5, 1910. 
II. 
Ehipicephalus appendiculatus (Bef. No. 364). 
The larvae originated from mothers collected in Natal on March 18, 
1910 ; the eggs w^ere laid at the laboratory on March 25th, and the larvae 
hatched out on April 26, 1910. The larvas were placed on Heifer 908 on 
August 12, 1910, viz., the 5th day after the rise of temperature, and were 
collected 5 days before death, which occurred on August 22, 1910, during 
which period the blood showed Theileria parva in large numbers, and in 
the spleen and glands plasma bodies were very frequently met with. 
The larvae moulted into nymphae on September 10, 1910. 
III. 
Ehipicephalus appendiculatus (Eef. No. 355). 
The larvae originated from mothers collected in Natal on March 18, 
1910, and the eggs were laid in the laboratory on March 25 ; the larvae 
hatched out on April 26, 1910. The larvae were placed on Heifer 913 
on July 14, 1910, at a time when the parasites of East Coast Fever were 
frequently met with, and the engorged larvae were collected from July 19 
to 21, 1910. Heifer 913 was killed on July 22, 1910. The examination 
of the blood since July 14th had shown the presence of the East Coast 
Fever parasites in but rare numbers ; free plasma bodies (agamogonous 
forms) had been noted in the glands. 
The larvae moulted into nymphae on September 1, 1910. 
IV. 
Ehipicephalus appendiculatus (Eef. No. 356). 
The larvae originated from mothers collected in Natal on March 12, 
1910. The eggs were laid at the laboratory on March 25th, and the larvae 
emerged on April 18, 1910. The larvae were placed on Heifer 914 on 
July 14, 1910, and were collected from July 17th to 20th. On July 18t1i 
Th. parva were noted in the blood of Heifer 914, and plasma bodies were 
seen in the glands. The heifer recovered. 
The engorged larvae moulted into nymphae on September 15, 1910. 
