Transmission of East Coast Fever by Ticks. 
329 
Y. 
Ehipicephalus appendiculatus (Ref. No. 349). 
The engorged nymphse were collected off sick cattle in Natal on 
June 29, 1910, and moulted in the laboratory on September 11, 1910. 
YI. 
Ehipicephalus appendiculatus (Ref. No. 411). 
The larvae originated from mothers collected in Natal on March 12, 
1910. The eggs were laid at the laboratory on March 14, 1910, and the 
larvae hatched on April 18, 1910. The larvae were placed on Heifer 914, 
suffering from East Coast Fever, on July 14, 1910. The engorged larvae 
were collected from July 17th to 20th and moulted into nymphae on 
August 15, 1910 ; they were placed on Heifer 1053 on October 18, 1910, 
at a time when this heifer was suffering from East Coast Fever and when 
Th. parva were noted in the blood and plasma bodies were seen in the 
glands. The engorged nymphae dropped from October 22 to 27, 1910, 
and moulted into adults on November 21, 1910. 
Note. — These ticks had been feeding in both their larval and nymphal 
stages on animals suffering from East Coast Fever. 
YII. 
Rhipicephalus appendiculatus (Ref. No. 426). 
The larvae originated from mothers collected in Natal on February 18, 
1910, and hatched by March 30, 1910 ; the larvae were reared on " clean " 
Heifer 868 and dropped from July 12 to 15, 1910 ; they moulted on 
August 23, 1910, and the nymphae were placed on Heifer 1111 on October 
26th at a time when this heifer was suffering from East Coast Fever. 
Th. yarva being found in the blood and plasma bodies in the glands. The 
engorged nymphae dropped from October 31 to November 5, 1910, and 
moulted into adults on November 26, 1910. 
YIII. 
Ehipicephalus appendiculatus (Ref. No. 373). 
The adult females originated from Cattle 906, 1088, 1009, and 1021, 
animals which were kept at the laboratory. The eggs hatched on 
November 18, 1910, and the larvae were placed on Heifer 909 on January 
2, 1911, at a time when this heifer was undergoing an attack of East 
Coast Fever and when Th. ijarva and plasma bodies were noted in the 
blood and glands respectively. 
