330 
Transactions of the Boyal Society oj South Africa. 
The engorged larvae were collected from January 5 to 7, 1911, and 
moulted about January 20, 1911, into nymphse. 
Testing of the Ticks, 
{a) Brown NYMPHiE (Eef. Nos. 363, 364, 355, 356, and 349). 
1. Heifer 627 had previously been used for inoculation experiments 
in connection with the transmission of East Coast Fever, but with 
negative results. 
Infested with brown nymphse (Eef. No. 363), on August 17, 1910 ; 
about 20 were found attached the following day, and the engorged 
nymphte were collected on August 23rd. No reaction ensued. 
Be-infested on September 15, 1910, with 20 brown nymphae (Eef. 
No. 364). All 20 ticks were found attached on the following day. 
No reaction followed. 
Be-injested on September 30, 1910, with brown nymphae (Eef. Nos. 
355 and 356). On the following day 14 ticks were found attached. 
No reaction ensued. 
Be-infested on October 20th with 6 brown adults, originating from 
Natal (Eef. No. 349). These adults were found fast on October 21st. 
The heifer contracted East Coast Fever and died on November 27, 1910. 
2. Heifer 911 had been previously used for inoculation purposes 
in connection with the artificial transmission of East Coast Fever, but 
with negative results. 
Infested on August 17th with a number of brown nymphae (Eef. 
No. 319). About 70 ticks were found fast the following day, and the 
engorged nymphae were collected on August 23rd. No reaction 
followed. 
Be-mfested on September 15th with brown nymphae (Eef. No. 364). 
20 ticks were found attached the following day. No reaction ensued. 
Be-infested on September 30, 1910, with brown nymphae (Eef. Nos. 
355 and 356). On October 1st, 20 ticks were found fast. No reaction 
ensued. 
Be-infested on October 20, 1910, by the infestation of 6 brown adults, 
origin Natal (Eef. No. 349). The heifer contracted East Coast Fever 
and died on November 6, 1910. 
3. Heifer 1014 had been previously used in connection with the 
artificial transmission of East Coast Fever, but with negative results- 
Infested on August 17, 1910, with brown nymphae (Eef. No. 319). 
24 ticks were found fast the following day, and the engorged nymphae 
dropped on August 23, 1910. No reaction resulted. 
