332 Transactions of the Boyal Society of South Africa. 
Be-infested on November 16, 1910, with 4 brown adults, origin Natal 
(Eef. No. 349). 3 adults were fast the following day. No reaction 
ensued. 
Be-infested on December 8, 1910, with 6 brown adults off East Coast 
Pever animal 1053 (Eef. No. 411), and re-infested six days later with 
2 brown adults off 1053 (Ref. No. 411) and 2 brown adults off 
Heifer 1111 (Ref. No. 426). 4 ticks of the first batch and 1 tick of 
the second batch were found fast on the day after infestation Ox 1046 
contracted East Coast Fever and died on January 8, 1911. 
{d) Beown nymphs (Ref. Nos. 363 and 364). 
7. Ox 1043 had been previously used for the artificial transmission of 
East Coast Fever, with negative results. 
Infested on September 5, 1910, with brown nymphse (Ref. No. 319). 
33 ticks were found attached the following day. No reaction resulted. 
Be-infested on October 20, 1910, with 10 brown nymphae (Ref. 
No. 364). 6 nymphae were fast the following day. Ox 1043 con- 
tracted East Coast Fever and died on November 18, 1910. 
8. Heifer 1017 had been previously used in connection with the 
artificial transmission of East Coast Fever, but with negative results. 
Infested on September 5, 1910, with brown nymphse (Ref. No. 363). 
42 ticks were found attached the next day. No reaction ensued. 
Be-infested on October 20, 1910, with 10 brown nymphae (Ref. 
No. 334). All 10 ticks were fast the following day. A fever reaction 
set in and Heifer 1017 died of East Coast Fever on November 9, 1910. 
(e) Rhipicephalus appendiculatus (Ref. Nos. 364, 349, 411, and 426). 
9. Heifer 1090 had not been used previously in any experiment, and 
was purchased in an area free from East Coast Fever. 
hifested on October 20, 1910, with 10 brown nymphae (Ref. No. 364). 
On the following day 6 nymphae were found fast, but no reaction 
developed. 
Be-infested on November 16, 1910, with 4 brown adults (Ref. No. 349), 
origin Natal. 2 adults were fast the following day, but no reaction 
ensued. 
Be-infested on December 8, 1910, with 6 brown adults off Heifer 1053 
(Ref. No. 411). 5 ticks were attached the following day. 
Be-i7ifested on December 14th with 2 brown adults off Heifer 1053 
(Ref. No, 411) and 2 adults off 1111 (Ref. No. 426). 2 adults were fast 
the following day. A reaction set in, and the animal died of East Coast 
Fever on December 27, 1910. 
