Transmission of East Coast Fever by Ticks, 
333 
(/) Ehipicephalus appendiculatus (Ref. Nos. 363, 364, and 349). 
10. Heifer 1082, an animal which had been bom in an area free from 
East Coast Fever and had not been used previously. 
Infested on August 30, 1910, with brown nymphse (Ref. No. 319). 
23 ticks were found attached on September 6, 1910, but no reaction 
followed. 
Be-infested on September 15, 1910, with 20 brown nymphse (Ref. 
No. 364). No reaction followed. 
Be-infested on October 20, 1910, with 6 brown adults, origin Natal 
(Ref. No. 349). On the following day 5 adult ticks were found fast. 
A reaction set in, and the animal died of East Coast Fever on 
November 25, 1910. 
(g) Rhipicephalus appendiculatus, nymphge (Ref. No. 364). 
11. Ox 1050 had been previously used in connection with the artificial 
transmission of East Coast Fever, without results. 
Infested on October 20, 1910, with 10 brown nymphse (Ref. No. 364). 
7 ticks were found attached the following day. A reaction set in, and the 
ox died on November 21, 1910, of East Coast Fever. 
Besidts. 
Rhipicephalus appendiculatus, nymphae (Ref. No. 319). 
These ticks failed to transmit the disease in every instance, and the 
9 animals which were infested were proved subsequently to be 
susceptible to East Coast Fever. The larvse hatched out on April 
26, 1910, and became infected at the end of May and beginning of June, 
that is, about 5 to 6 weeks after they had hatched. They moulted into 
nymphae on August 5th, viz., about 2 months after they had become 
replete on Heifer 1013. The shortest moulting period in this stage 
is 16 days in the summer. They were placed on the susceptible animals 
as follows : — 
Heifer 627 infested on August 17, 1910 20 ticks were fast 
911 
1014 
1068 
1082 
1037 
1046 
1017 
1043 
70 
24 
70 
August 30, 1910 20 
September 5, 1910 27 
28 
42 
33 
