Transmission of East Coast Fever by Ticks. 
335 
Khipicephalus appendiculatus, adults (Ref. No. 349). 
These ticks transmitted the disease to five animals out of seven. The 
engorged nymphse were collected off sick cattle in Natal on June 29, 1910, 
and moulted into adults at the laboratory on September 11, 1910, or 
74 days after collection. 
They were used for the infestation of — 
Cattle 627 on October 20 
911 
1014 
1068 
1082 
1046 
1090 on November 16 
1910 3 became attached 
6 
6 
5 
5 
3 
1910 2 
Rhipicephalus appendiculatus, nymphse (Ref. No. 373). 
These ticks transmitted the disease to one animal (1037). As larvae 
they were infected on Heifer 909 on January 7, 1910. They moulted into 
nymphae on January 20, 1911, and were used on January 30, 1911, when 
10 days old. 
They were placed on — 
Heifer 1037 on January 30, 1911, and 20 became attached. 
Rhipicephalus appendiculatus, adults (Ref. Nos. 426 and 411). 
Batch No. 426 transmitted the disease to two animals, 1040 and 1090. 
They were infected as nymphaR on October 26, 1910, and moulted into 
adults on November 26, 1910. 
Batch No. 411 were infected as nymphae on October 18, 1910. They 
moulted into adults on November 21, 1910. 
The adults of batch 426 were 18 days old when used, and the adults of 
batch 411 were 18 days old when used. 
Ticks Ref. No. 411 were placed on Heifer 1046 on December 8, 1910, 
of which 4 became attached, and on December 14th Ref. Nos. 411 and 426 
were infested, of which 1 became attached. Ref. No. 411 undoubtedly 
transmitted the disease. Ref. No. 411 were also placed on Heifer 1090 on 
December 8, 1910, of which 5 became fast, and on December 14, 1910, 
411 and 426 were infected, of which 2 became fast. The last batch 
transmitted the disease. 
