230 
CHARLES O. APPLEMAN 
Table VI 
Effect of Greening on Respiration, Catalase and Oxidase 
Experi- 
ment 
Milligrams of CO2 per 
Kilo per Hour 
Manometer Readings Expressed in Centimeters of Mercury 
Catalase 
Oxidase 
Normal 
Green 
Normal 
Green 
Normal 
Green 
I 
2 
17.14 
14.32 
19.50 
16.24 
+ 2.5 
+ 2.1 
+ 2.8 
+ 2.5 
- 2.35 
— 2.2 
— 2.1 
— 2.1 
Effect of Sprouting: Whole tubers of many varieties produce sprouts 
only from the buds on the seed end. Tubers of such varieties were 
cut in half and respiration measured separately in the seed and stem 
halves. It was found that respiration is always much higher in 
the seed halves when the sprouts are left on. This difference seems 
to be greater during incipient sprouting than after the sprouts have 
attained considerable size. How much of the increased respiration in 
the seed ends bearing the sprouts is due to the respiration of the 
sprouts themselves is difficult to determine. If the sprouts are re- 
moved just prior to the measurement of respiration, the results are 
quite different and depend upon the variety in question. In all cases 
the difference in respiration between the seed and stem ends is sud- 
denly reduced. In McCormick tubers, it always remains a little 
higher in the seed halves. 
The behavior of the catalase activity in the two ends of the McCor- 
mick tuber is practically identical with that of respiration. No 
difference in the oxidase activity of the two ends could be detected 
by the Bunzel method, using either pyrocatechin or pyrogallol as the 
oxidizable substance. 
Table VII 
Comparison of Respiratioti in Seed and Stem Ends; McCormick Tubers, Sprouting 
Only From Seed Ends 
Sprouts Seed Ends Stem Ends 
Not Started 100 93.5 
Not started lOO 93.2 
On during measurement 100 58.2 
Removed prior to measurement 100 
Removed prior to measurement 100 
The results of a large number of colorimetric determinations, using 
aloin as the oxidizable substance, show that this method does not 
