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Transactions of the Royal Society of South Africa. 
number of other substances have been tested with ox's corpuscles treated with 
brilliant green to ascertain whether other proteins, apart from those of 
serum, possess this property ; varying amounts of egg albumen were added 
to ox's corpuscles which had been treated with different amounts of brilliant 
green. No haemolysis resulted. Peptone and gelatin solutions were also 
tested and found inactive. Other bodies which have been shown to play 
a part in various haemolytic phenomena were also tested. Thus varying 
amounts of brilliant green were added to ox's corpuscles which had been 
sensitised with 5 M.H.D. of a powerful haemolytic immune body (0 0025 
to 1 c c.) without effect. Colloidal silicic acid also showed no power of 
lysing corpuscles treated with brilliant green. The addition of varying 
amounts of brilliant green to venomised corpuscles also failed to produce 
lysis. Grlucose solution had no haemolytic action on corpuscles treated with 
brilliant green. 
Human cerebro- spinal fluid was found to be actively haemolytic to ox's 
corpuscles sensitised by brilliant green, though comparatively large amounts 
were necessary (0"1 c.c. to 0'25 c.c.) ; cerebro-spinal fluid was also found to 
have no inhibitory action on the lysis by the serum and brilliant green. 
Cerebro-spinal fluid heated for five minutes at 100° C. was as active as fresh 
cerebro-spinal fluid. This lysis occurred even when the fluid contained a 
minimal amount of protein, as evidenced by the absence of a precipitate or 
turbidity on the addition of an equal volume of 96 per cent, alcohol. These 
were all of course pathological cerebro-spinal fluids removed from cerebral 
and spinal cord cases for the Wassermann test. There was no correspon- 
dence between their activity with brilliant green and their power of reacting 
in the Wassermann test. Nos. 1 and 2 (Table XIII) contained small amounts 
of protein (shown by the alcohol test) ; No. 3 contained no appreciable 
amount of protein, and it was the most active, showing clearly that the 
effect does not depend on the presence of the serum proteins in the fluid. 
The fluid from a cystic swelling of the axilla was tested with corpuscles 
sensitised with brilliant green. This fluid was highly albuminous, and small 
quantities were sufficient to produce lysis (Table XIII). A richly albuminous 
urine was also tested. The albumin of 20 c.c. was precipitated by alcohol, 
removed by centrifugalisation, and suspended in 3 c.c. 0*85 per cent. 
NaCl. It was found that 0-5 c.c. of this suspension was capable of pro- 
ducing lysis of ox's corpuscles treated with brilliant green (Table XIV). 
The susceptibility of ox's red blood-corpuscles to lakingby hypotonic salt 
solution, acid and alkalies was investigated. Thus it has been shown that 
in certain pathological states, alterations occur as regards the susceptibility 
of human erythrocytes to various laking agents (McNeil and others) (5). 
It was found, however, that ox's corpuscles treated with brilliant green 
exhibited the same degree of susceptibility to hypotonic salt solution as 
normal corpuscles. 
