238 
TODD AND WOLBACH. 
the kidney showed a slight turbidity in glycerine broth and some growth 
on the blood serum and on the glucose agar. Plates from this material 
showed the presence of microscopic colonies of a diplococcus which 
stained by Gram's method. A similar organism was present in the 
lymphatic glands. The attempts to grow this organism in broths of 
various kinds failed ; it would not live for more than four days in culture 
media. 
Horse II. —With the exception that the culture media containing 
blood were prepared with blood drawn off a few hours previously from 
this animal, the routine followed in making the cultures from the Black 
Gelding was followed in making cultures from this horse. 
With the exception of a few colonies which could be attributed to aerial 
contamination from the stable, the results obtained were entirely negative. 
Cultures on blood peptone agar, beef peptone agar, and water agar 
were carefully examined with high magnifications at frequent intervals 
for a fortnight without result. 
At the autopsy of this horse culture tubes were again inoculated in the 
same way as before. Three organisms, none of them pathogenic to 
guinea-pigs, were isolated. Two were unidentified ; one was Micrococcus 
varians lactis. # 
It is of interest that this organism has been met with on several occa- 
sions at Macdonald College in the organs of animals. In one instance it 
was isolated from the udder of a virgin heifer. 
XIII. Summary. — The symptoms which were constant in 
our infected horses were : an irregular temperature, accom- 
panied by weakness, emaciation and anemia of varying 
severity; edemas rarely occurred and petechiae of the mucous 
membranes were not seen. When death occurred it followed 
in from four to eight months after the appearance of symp- 
toms. One horse, which has possibly recovered from the 
disease, is still healthy sixteen months after the appearance 
of the disease. 
At the autopsies, emaciation was the most constant feature. 
The spleens were markedly enlarged * and, sometimes, con- 
gested ; in two instances their capsules were dotted with 
petechial hemorrhages ; sub-serous hemorrhages, other than 
these, were not seen. With the exception of an infarct of 
the lung in Horse II. and of microscopical extravasations 
*The normal weight of the spleen of a horse of the weight of the Black Gelding is 
about two and a half pounds. 
