THE SWAMP FEVER OF HORSES. 
weeks they were examined daily in fresh or stained prepa- 
rations ; no visible bacteria nor other parasites were seen in 
them. 
On April 2d blood was taken from the jugular vein of 
Horse IV. (temperature 103.2^ F.). A mixture containing 
.2 per cent of sodium chloride and another mixture contain- 
ing .3 per cent of sodium citrate was centrifugalized. Four 
hanging-drop preparations were made of the white layer 
from each mixture; these preparations were kept at 37^ C. 
and carefully examined at intervals during four days; no 
parasites nor bacteria were seen in them. Four tubes, two 
containing five cubic centimeters of the sodium chloride 
mixture and two containing five cubic centimeters of the 
citrate mixture were kept at room temperature ; four other 
such preparations were kept at 37^ C. All were examined 
at intervals during four days ; no parasites nor bacteria were 
seen in them. 
Quantities of blood, varying in amount from two cubic 
centimeters to five hundred cubic centimeters, were taken 
from the Black Gelding and from Horse IV. ; these were 
kept at room temperature or at 37° C. They were examined 
constantly for a fortnight ; no bacteria nor other parasites 
were seen in them. 
Many observers (Peters, Torrance and Bell, Mack) have 
unsuccessfully attempted to cultivate a specific organism 
from the body fluids and tissues of cases of Swamp Fever. 
Dr. F. C. Harrison, Professor of Bacteriology at Macdonald 
College, attempted to cultivate an organism from our cases ; 
his efforts were also unsuccessful; they are briefly recorded 
below : 
Black Gelding. — Material for inoculating the culture media was taken 
from a vein in the leg, a vein in the abdomen, from the lymph duct, from 
the pericardium, from the spleen, from the kidneys, lungs, brain, and 
from the bladder. Cultures on the following media were made with each 
material: beef broth, glycerine broth, beef peptone, beef peptone agar, 
beef peptone glucose agar, glycerine agar, milk, coagulated blood serum, 
and urine. 
The cultures made from the lung were contaminated. The culture 
tubes from the brain were also contaminated. The cultures made from 
