236 
TODD AND WOLBACH. 
stained by Gram's method and by iron hematoxylin, and bear some 
resemblance to Leishman's bodies; this is particularly so in specimens 
stained by Gram's method. 
There is much dark brown pigment scattered throughout the liver cells 
and a smaller amount of paler brown perinuclear pigment. 
The portal spaces are markedly infiltrated with lymphocytes. 
The liver, as a whole, is very similar to that of the Black Gelding, but 
the post-mortem changes are too great for a careful cytological study. 
Kidney : Congested. Glomeruli normal. There is very slight lympho- 
cytic infiltration of the interstitial tissue. There is probably slight cloudy 
swelling but the state of the tissues makes a reliable report impossible. 
Case V. (Dr. Ballah). — Post-mortem changes are marked. Lung: 
Normal. Spleen: There is extreme hyperemia. The Malpighian bodies 
are very small. The structure of the pulp is not distinguishable, owing to 
the blood and, possibly, also, because of post-mortem changes. There are 
many large cells containing dark brown pigment in the pulp. Liver: 
There are many small necroses similar to, but less extensive than, those 
seen in the preceding case. The pigmentation is also less marked and 
the ring bodies, though present, are less conspicuous. Kidney : The 
glomeruli are normal. There is marked perivascular and interstitial 
infiltration with lymphoid and plasma cells. Slight cloudy swelling. 
Lymph glands : Both sections show congestion. The sinuses contain 
blood and many large phagocytic cells containing red blood corpuscles 
and pigment. 
A portion of a liver, taken from a horse which died from Swamp Fever, 
was sent to us by Dr. Torrance of Winnipeg. Sections were made of 
it and examined. The appearances seen were similar to those observed 
in the livers of our own cases. 
XII. Cultures. — The fact that Leishmania will develop 
conspicuous flagellating forms in tubes containing infecting 
material, diluted with sodium citrate, and kept at room tem- 
perature, suggested that blood from our horses should be 
examined in the same way. 
On January 23, 1910 (temperature 96° F.), blood was 
taken from the Black Gelding; two cubic centimeters of it 
were placed in each of one dozen tubes and the blood was 
diluted with solutions so as to give tubes containing a mix- 
ture of blood and from .5 per cent to .12 per cent of sodium 
citrate; other tubes contained a mixture with from 1.4 per 
cent to .35 per cent of sodium chloride. These tubes were 
placed in an incubator at 37° C. and for two and a half 
