THE SWAMP FEVER OF HORSES. 
233 
original horses and from tlie horses (Nos. I., II., III., IV., 
and V.) inoculated from them. 
* Large quantities of blood were always used ; rats often 
received ten cubic centimeters and the kittens as much as 
thirty cubic centimeters; other animals received propor- 
tionate amounts. So far as it was possible the blood for 
inoculation was always taken from the horse while its tem- 
perature was higher than normal. 
No symptoms, such as emaciation and rise of temperature, 
which might be attributed to Swamp Fever, followed the 
inoculation of any of these animals. Four of the rats and 
two guinea-pigs which received blood from the Black Geld- 
ing died, from no apparent cause, about two months after 
the inoculation. Some of the animals died of intercurrent 
infections, such as pneumonia; others were killed in order 
that they might be examined. The remainder of the ani- 
mals are still living and healthy. Careful post-mortem 
examinations were made of all the animals that are dead ; 
no constant lesions, either macroscopical or microscopical, 
have been found in any of them ; in one or two the spleen 
was slightly enlarged ; in one dog the abdominal lymphatic 
glands were much enlarged and hemorrhagic. Very marked 
anaphylactic phenomena, such as vomiting, purging, dysp- 
nea and, at the autopsy/, edema of the lungs and conges- 
tion of the liver, were noted in puppies, rabbits, and cats. 
The symptoms were observed at the first, second, and third 
repetitions of inoculations of horse blood into animals 
which had previously received doses of blood from either 
the same or from another horse. The first doses of blood 
given measured about ten cubic centimeters; the last ones 
about thirty cubic centimeters for each animal. 
XI. Observations on Cases and on Sub-inoculations. — 
The blood of the original hgfrses and of all of the experimental, 
animals was examined very carefully and almost daily, either 
in coverslip preparations or in stained films; and from time 
to time ten cubic centimeters or more of blood from the 
horses was centrifugalized and examined. 
