44 
HEMOLYMPH NODES OF THE SHEEP 
red cells were found nowhere, however, save in foetal nodes. Although 
the erythrocytes were generally well preserved, all gradations between per- 
fect cells and small masses of cell detritus were found in specimens pre- 
pared in many different ways. As might be expected, however, they were 
frequently well preserved in- nodes depleted of lymphatic tissue ; although 
the absence of fragments of erythrocytes within these nodes may have 
been due, in part at least, to the freer circulation which must exist in 
them and which assures a freer drainage of the node. In some specimens 
which were only partially rilled with lymphocytes and blood, many eryth- 
rocytes assumed most unusual shapes, which probably cannot be attrib- 
uted to poikilocytosis. In some Zenker preparations, for example, many 
isolated cells which were not crowded upon by others were truly cup- 
shaped, as has been variously observed. The outlines of others were 
ovoid, or crescentic; while still others looked as though the cell mem- 
brane had ruptured, and retracted somewhat, thus leaving an open- 
ing with an irregularly torn margin. Among these cup-shaped cells some 
fragments of erythrocytes were always found; but most of them stained 
no differently than the normally-shaped ones. Aside from this disinte- 
gration, the erythrocytes, particularly in nodes depleted of much of the 
lymphatic tissue, also exhibited some variability, in staining characters. 
In Zenker preparations, most of them usually stained a golden yellow with 
orange G ; but groups or areas of others took the pink of Congo red or 
eosin ; and practically all gradations in staining reactions were found be- 
tween these colors. The best-preserved cells usually stained with orange 
G, as was the case in embryonic nodes ; although it must be added that 
irregularly shaped erythroblasts contained in the latter, occasionally 
stained pink. Since, however, unmistakable fragments of erythrocytes 
also often stained pink, it may be inferred that this selective staining 
quality on part of some erythrocytes probably signifies little else than in- 
cipient degeneration. Since some of the fragments of erythrocytes were 
circular in outline, they simulated blood platelets so closely that if found 
in circulating blood they would in ordinary preparations probably be in- 
distinguishable from them. Although it was sometimes difficult or im- 
possible to stain the erythrocytes in the blood spaces satisfactorily, it is 
doubtful whether this fact can be attributed to a deficiency in hemaglobin, 
as held by Meek [17], in case of cells from pathological glands taken from 
the human body. 
Polymorphonuclear leucocytes were common, especially in some parts 
of the parenchyma and in the veins. They were rather uncommon in the 
blood islands and in the peripheral blood space, and were not observed 
