Brown Institution on Pleuro-Pneumonia. 165 
APPENDICES. 
I. Exposure of animals to emanations from diseased organs. 
I[. Subcutaneous injection of pleuro-pneumonia exudation-liquid. 
III. Inoculation in the tail by the ordinary method. 
IV. Fatal case of "blood poisoning" apparently consequent on infusion 
of pleuro-pneumonia liquid into the circulating blood. 
V. Inoculations by infusion into the veins. 
VI. List of animals experimented upon. 
APPENDIX I. 
Exposure of Animals to the Emanation from diseased organs. 
Sept. 9, 1876. — Portions of lung from a cow affected with pleuro-pneumonia 
were conveyed direct from a slaughter-house to the Brown Institution, and 
while still warm were placed in the mangers of two two-year-old bullocks, 
known as animals Nos. 3 and 4 in this Report. The diseased material was 
allowed to remain in the mangers during the night, and removed in the 
morning along with a considerable amount of serous fluid which had drained 
from it. No washing or disinfection was done after its removal. Both these 
animals were kept under close observation, and no result of this exposure was 
observed, and no rise of temperature was recorded. 
March 27, 1877. — The above experiment was repeated by placing portions 
of diseased lung in the mangers of a cow, about six years old, and a two-year- 
old bullock, known as animals Nos. 2 and 5 in this Report. In this experi- 
ment the portions of lung were allowed to remain for twenty-four hours, and 
when removed the mangers were neither washed nor disinfected. It should 
be noted that in the case of No. 5 he had barely recovered from an attack of 
foot-and-mouth disease, communicated by means of saliva obtained from 
diseased animals at the Metroix)litan Cattle Market. Neither of these 
animals was affected by this contact with the pleuro-pneumonia virus, and 
the daily recorded temperature remained normal. 
August 16, 1878. — A favourable opportunity presented itself for repeating 
tliis experiment on a larger scale, when portions of fresh diseased lungs were 
placed in the mangers of seven animals, two cows and five yearlings, known 
as animals Nos. 10 to 15 in this Report ; these animals did not suffer from an 
exposure of twenty-four hours to these diseased organs. 
APPENDIX II. 
Injection of Pleuro-Pneumonia Exudation-liquid into the Cellular 
Tissue. 
September 6, 1878. — Five yearling heifers which had been kept under daily 
observation at the Brown Institution since March, and had been the subjects 
of experiments on anthrax, were inoculated by the injection of a few drops 
of the liquid obtained from a portion of diseased lung (taken from a cow 
slaughtered the same morning) into the subcutaneous cellular tissue on the 
side of the neck and front of the shoulder. 
The punctures could be felt as mere prominences on the following day, 
and continued so in all the animals for four days. On the fifth day, in one 
there was some swelling and tenderness at the seat of puncture, and on the 
following day a similar appearance was observed in another. The daily record 
kept will best show the progress and the result of this swelling. 
Si2)t. 11, 1878. — At the seat of puncture iu No. 14, there is a little doughy 
paiuiul swelling. 
