168 
Concluding Report on the Experiments at the 
APPENDIX III. 
Inoculation in the Tail by the ordinary Method. 
A healthy cow, about six years old, which had been kept under "daily 
observation for several months, and had on three occasions during the month 
of June, 1876, resisted infection with foot-and-mouth disease, calved on 
August 15, 1876, and for a few days both before and after calving an increase 
in the bodily temperature was noted. As determined by a long series of 
observations, the mean normal temperature was 101*6 Fahr., the range of 
readings being from 101 to 102-2. 
Nov. 3, 1876. — About two drops of the fluid which had drained from a 
diseased lung were injected into the cellular tissue of the tail about 3 inches 
above its termination. For the first four days the seat of the puncture 
could be felt as a very small prominence, but on the fifth day it was tender 
and slightly swollen. The swelling slowly increased up to the 10th day, 
and was at that date confined to the seat of puncture, and about 1| inch in 
diameter. 
On the 12th day a slough had formed in the centre of this swelling, which 
separated on the ] 7th day, and left an ill-conditioned sore. 
A much more extensive secondary swelling now began, involving the whole 
circumference of the lower part of the tail, and spread gradually upward for 
9 inches above the seat of the inoculation by the 33rd day. At this time about 
12 inches of the lower part of the tail was twice its natural thickness. This 
swelling was firm, cold and painless, except at its upper margin, where it 
terminated somewhat abruptly in a narrow band, where the skin was hot 
and tender, and, at which point by the 38th, softening began, and the future 
line of separation was marked out. The dead portion of the tail gradually 
shrivelled, and finally separated without haemorrhage on January 3, 1877, 
the 58th day after inoculation. Under ordinary circumstances ' the dead 
portion of the tail would have been amputated long before, but in this case 
the sequel to the inocixlation was watched without interference. 
While these changes were taking place at the seat of inoculation the animal 
showed no signs of constitutional disturbance ; the mean temperature during 
the 58 days was 101'64, and the daily readings ranged from 101"2 to 102"2. 
No suppuration attended this sloughing, and the small raw surface on the 
end of the stump healed in a few days. 
This animal was afterwards placed in a cow-shed where pleuro-pneumonia 
existed on November 23, 1877, and kept there until January 9, 1878, when, 
being so ill that there was little prospect of recovery, she was slaughtered. 
A post-mortem examination showed that the illness of this animal was 
due to the presence of a large surgical needle which had been swallowed 
with the food, and having transfixed the wall of the second stomach had 
injured the liver and diaphragm, causing a large diaphragmatic aljscess; 
in addition, the liver was extensively diseased and contained a large number 
of parasites. The lungs and pleura were ix;rfcctly healthy. No trace of any 
adhesions or sub-pleural exudation. 
APPENDIX IV. 
Fatal case of " Blood poisonincj" apparently consequent upon the injection 
into the circulating blood of Pleuro-Pneumonia Virus. 
A cow sujiposed to be about tliirteen years old had been kept under daily 
observation at the Brown institution for eight mouths. In June, 1876, she 
