Brown Institution on Pleuro-Pneumonia. 171 
drawal of the cannula was found sufficient to stop any slight bleeding from 
the puncture, and thus the trouble and danger of applying a ligature to the 
vein was found unnecessary. 
Sept. 9, 1876. — The animals, known in this Eeport as Nos. 5, 6, 7 and 8 
(see Appendix VI.), had been under daily observation at the Brown Insti- 
tution for three months, and during the whole of that time had remained 
perfectly healthy, as is shown by the following tem^jeratures : — 
Highest. Lowest. Mean. 
No. 5.— 102-6 .. 101-45 .. 101-65 
„ 6.— 102-6 .. 100-4 .. 101-55 
„ 7.— 102-8 .. lOri .. 101-95 
„ 8.— 102-6 ., 101-2 .. 101-9 
The inoculation was perfoi-med by injecting two fluid-drachms of fresh pleuro- 
{ineumonia exudation into the vein. The only local effect observed was a 
ittle swelling of the wound, which disappeared, leaving only the cicatrix of 
the incision by the fourteenth day. 
No constitutional disturbance followed this injection of the virus into the 
blood ; the daily temperature of all the animals continued normal, as is shown 
by the following Table, the results of forty daily observations of each animal : — 
Highest. Lowest Mean. 
No. 5.— 102-6 ,. 101-4 .. 101-65 
„ 6.— 102-2 .. 101-3 ,. 101-71 
„ 7.— 102-9 ., 100-7 .. 101-8 
„ 8.— 102-4 .. 101-0 101-7 
Nov. 3, 1876. — Some of the fluid collected for the inoculation of No. 2 
in the cellular tissue of the tail was used for infusion into the vein in animals 
Nos. 3, 4 and 9 (see Appendix VI.). The animals Nos. 3 and 4 had been 
exposed to contact with portions of diseased lung on Sept. 9, but the daily 
temperature recorded commencing in June, shows that they had been healthy 
during the whole time ; — 
Highest. Lowest. Mean. 
No, 3.— 101-9 „ 101-1 .. 101-29 
„ 4.— 102-0 ,. 100-0 .. 101-15 
In the case of No. 9, the animal was barely three months old, and the 
normal temperature, as the mean of twenty daily observations, was found to 
be 102-2. 
The local effect produced by the injection into the vein was only slight 
in Nos. 3 and 4, and the wounds had closed and all swelling disappeared in 
from twelve to sixteen days. In the case of No. 9 the vein was so small that 
even when fully exposed some difficulty was found in introducing the cannula. 
Although no infiltration of the surrounding cellular tissue resulted from 
the injury inflicted, an abscess about the size of a pigeon's egg formed, and 
the wound was not closed until the twenty-third day after the inoculation, 
but no secondary swelling appeared, and no general disturbance was observed 
in any of these animals, even although the quantity of fluid injected was 
three fluid drachms. As proof of this, the result of the dailj' temperature 
observations, during the forty days immediately succeeding the operation, may 
be quoted : — 
Highest. Lowest. Average. 
No. 3.— 102-4 .. 101-2 .. 101-7 
„ 4.— 102-0 ., 101-2 .. 101-6 
„ 9.— 102-8 .. 101-9 ,. 102-21 
Dec. 12, 1876. — A portion of the material used for the inoculation of the 
cow No. 1, referred to in Appendix IV., was used for the second inoculation of 
