172 Concludivg Reports on the Experiments at the 
Nos, 3, 4, 5 and 6. The operation was this time performed without making 
an incision in the skin. The local effect produced by the puncture was only 
a very slight prominence, which could be felt for a few days. 
The quantity of fluid injected was again three drachms. 
No constitutional effects were visible, as the result of this inoculation, 
although the animals were under observation for some months, as will be seen 
by reference to Appendix VI. 
They were afterwards submitted to contact with diseased animals, and 
were kept in infected places, to test, as far as practicable, the protective value 
of the inoculation. The animals Nos. 3 and 4 were slaughtered March 1st, 
1878, after a test exposure in an infected shed in November and December, 
1877. A post-mortem examination was made, and the lungs of both were 
found in every respect healthy. Animal No. 5 was infected with foot-and- 
mouth disease in March, 1877, and was placed in a shed infected with ])leuro- 
])neumouia, in November, 1877, and continued healthy up to the end of March, 
1878, when he was disposed of. No. 6 was placed among infected stock, July, 
1877 ; was brought back to the Brown Institution in February, 1878, when he 
was made the subject of a test experiment relating to the communication of 
anthrax by feeding. 
March 27, 1877. — Animals Nos. 7, 8 and 9 were a second time inoculated 
by injecting into the veins three drachms of pleuro-pneumonia virus. 
Since their previous inoculation on iSeptember 9, 1876, Nos. 7 and 8 had 
been castrated in November, the result of which operation was to cause an 
increase of temperature for a few days. They had also been affected with 
foot-and-mouth disease in February, 1877, induced by eating hay moistened 
with the saliva of diseased animals. 
As the result of this pleuro-pneumonia inoculation, no general effect was 
produced; they were all kept at the Brown Institution till July, and then 
placed along with No. 6 among diseased stock, and brought back to the 
Brown Institution, for experiments on the production of anthrax in February, 
1878. Nos. 7 and 8 were afterwards sold at the end of March, but No. 9, 
for which another animal was obtained in exchange, remained at the Brown 
Institution in a healthy condition till the middle of May. 
September 2, 1878. — The five yearlings referred to in Appendix II. were 
inoculated by the injection of two drachms of pleuro-pneumonia virus into the 
vein. At the seat of puncture a mere prominence could be felt, which had 
quite subsided by the 6th, when the subcutaneous injection was performed. 
'I'hese animals had been the subjects of anthrax experiments in May and 
June, but their temperatures had remained normal from the middle of July, 
and continued so until after the subcutaneous inoculation on September 6. 
As no opportunity was found for placing these animals in infected sheds, 
they were finally sold in a healthy condition in November. 
Septeinher 6, 1878. — Two cows, which had been under almost daily 
observation at the Brown Institution during Maj% June, and July, while the 
subject of vaccination observations, not connected with the work of the 
Koyal Agricultural Society, were purchased for pleuro-pneumonia experiments. 
Some of the pleuro-pneumonia virus used for the subcutaneous inoculation 
of the five yearlings, Nos. 10 to 14, was injected into the vein of these two 
cows. No result was observed further than a mere prominence at the seat of 
puncture, which could be felt for a few days. 
As these could not be placed in infected places, they were disposed of as 
healthy in the beginning of November. 
