450 Report of Experimental Work on Protective 
ment as a preventive for the disease of the Pasteurian prepared 
" vaccine." 
On October 13th, 1886, some tubes of Anthrax vaccine 
were obtained from M. Pasteur's agent in Paris. On the 
same day the specified dose of " premier vaccin " was injected 
into the subcutaneous tissue of two young bullocks at the 
College. 
Up to the 24th there was no appreciable systemic disturbance. 
The temperature remained normal. At this date each animal 
was inoculated with the prescribed dose of " deuxieme vaccin." 
As little disturbance followed this latter part of the operation 
as was noticeable after the former. (It should be mentioned that 
two inoculations, with an interval of ten days between each, are 
deemed necessary for the provision of protection.) This vaccine, 
microscopically examined and cultivated, was found to contain 
the anthrax bacillus. At the time of the last protective inocu- 
lation a small quantity of " deuxieme vaccin " was injected 
into a rabbit and guinea-pig. On the following day both these 
small creatures were found very unwell. Sixty-four hours aft?r 
inoculation the guinea-pig was found dead, and 108 hours afte: 
inoculation the rabbit. The blood of both contained anthrax 
bacilli in considerable numbers. A yellow mouse, inoculated 
with a drop of blood from the dead guinea-pig, died in 36 
hours. 
Cultivation with blood from these several sources verify the 
statement that in Pasteur's "deuxieme vaccin" there are bacilli 
capable of killing the rabbit, guinea-pig, and mouse, but which 
in due time, after inoculation with "premier vaccin," have no 
deleterious effect on young bovines. 
We now experienced some difficulty in obtaining unattenuated 
virus from a naturally contracted case of anthrax. It was not 
tin November 29th that we received from Dartford an affected 
spleen, about the condition of which for experiment there might 
be some doubt. Its virulence was, however, proved by inocu- 
lation of a rabbit, which died 21 hours after, its blood swarming 
Avith the anthrax bacilli. Cultivation yielded a fine growth in 
24 hours, some of which injected into another rabbit induced 
death in 32 hours. 
On December 10th, some viscera were obtained from another 
fatal case of anthrax in a bullock. Microscopic examination 
of spleen pulp showed anthrax bacilli in large numbers. It was 
decided to use this as a test. About 30 drops of a mixture 
of spleen pulp and distilled water, were injected into the sub- 
cutaneous tissue of one of the oxen " protected " with Pasteur'a 
vaccine, and of an ox which liad not been so protected. There 
was no appreciable general indisposition in either case. The 
