454 Report of Experimental Work on Protective 
1886, the first reliable material (some fresh muscle-juice) from 
a naturally contracted case, and expeditiously despatched in 
bulk to us, by Mr. Clark, M.R.C.V.S., of Horncastle. Exami- 
nation showed the characteristic bacilli. 
At 3.45 r.M., one drop of muscle-juice was injected into the 
subcutaneous tissue of each of two guinea-pigs. Five drops 
from the same source were injected into the muscles of the 
haunch of a young bullock of about ten months. 
At 6 A.M. on the 4th, one guinea-pig was ill, the parts about 
the seat of inoculation showing characteristic emphysematous 
swelling ; at 4 p.m., the same conditions were manifest in the 
other guinea-pig. At 10 A.M. (4th), the bullock's health 
appeared much disturbed, temperature 105 "7, the limb much 
swollen. A course typical of Quarter III terminated fatally in 
case of one guinea-pig 18^ hours after inoculation, of the other 
guinea-pig in 36 hours, and of the young bullock in 57J hours. 
Post-mortem examination revealed positive evidence of the 
disease. 
Microscopic inspection and sowings of both blood and muscle- 
juice from each of the above cases were made. In no instance 
could bacilli be detected in the blood, while in variable numbers 
they were always seen in muscle-juice. Cultivation of muscle- 
juice in veal broth never failed to yield growths of characteristic 
bacilli. Sowings of blood under same circumstances were 
invariably sterile. 
With muscle-juice from another very malignant case reported 
to us on November 11th we inoculated two guinea-pigs, which 
died respectively 34 and 84 hours after inoculation. Soon after 
death of that which survived only 34 hours, two other guinea- 
pigs were inoculated with its blood. These animals seemed to 
suffer little or no inconvenience, and lived under our observation 
for two months. * 
Cultivations ol serosity taken from the above young bullock 
12 hours prior to death (from Quarter 111) by puncturing with 
a glass pipette showed bacillar growth in 24 hours similar to 
that of muscle-juice of guinea-pigs inoculated at the same time. 
The spores were, however, very numerous. Inoculation of 
rabbits with this material did not produce the disease. 
The point of inoculability of the disease, and its identity 
with tndt described by Continental writers, appear from the 
foregoing to be satisfactorily established. 
Our investigations into methods for obtaining protection 
from the disease have been prosecuted on principles enunciated 
by three eminent French veterinary surgeons, MM. Arloing, 
Cornevin and Thomas, of the Lyons Veterinary School, in a 
work published in 1883. These savans describe measures, 
