Inoculation for Anthrax and Quarter III. 
455 
all of which they state to be effectual in inducing a state of 
immunity, by the introduction into the healthy animal of the 
virus in its natural state or in a state of attenuation, 
j Discarding the others as less practical, we have directed 
our experiments mainly to two plans apparently more easy of 
application in this country. From public report it would appear 
that in France " vaccination " for Quarter 111 is chiefly carried 
out (and this extensively) by introduction of dried muscle-juice 
into the subcutaneous tissue. The great advantages claimed 
for this method over most others are that the material ready for 
use may at any time be obtained by the veterinary surgeon, and 
that in its proper use there is not the slightest danger to the 
vaccinated animal. The " vaccin " is in France now regarded 
as an article of commerce, and having first ascertained by letters 
from M. Arloing that M. Fromage of Paris was his agent for the 
sale of it, we obtained from this source a supply of material. 
Through the kindness of C. De Murrieta, Esq., of Wadhurst 
Park, Sussex, who, in August, 1886, wrote expressing his great 
interest in the matter, and generously placed some of his young 
stock at our disposal, we were enabled to commence testing the 
efficacy of Arloing's dried muscle-juice. 
On August 21st, Professor Penberthy proceeeded to Wad- 
hurst Park, and carried out the first part of the process of 
protective inoculation by injecting into the subcutaneous tissue 
of the tails of six young beasts (carefully marked for distinction) 
a few drops of a solution in pure water of Arloing's prepared 
muscle-juice subjected to a temperature of 100° C. After an 
interval of ten days. Professor Robertson proceeded to Wadhurst 
Park and completed the process by injection in the same situ- 
ation of the vaccine No. 2, which is dried muscle-juice subjected 
to a temperature of 85° C. 
These animals were then, with six others unprotected, placed 
in a pasture traditionally notorious for the fatalities from Black 
j Quarter occurring in it. Notwithstanding the assurances of the 
I attendants acquainted with the place, that some, if not all, 
would be attacked, two months elapsed without the slightest 
: appreciable change in the animals, save a little soreness at the 
1 seat of inoculation. Temperatures regularly taken and carefully 
I registered by Mr. Chapman, agent at Wadhurst, showed little 
variation. 
It was determined to test further the efficacy of this operation 
^ by introduction of muscle-juice from a naturallv contracted case, 
1 The first opportunity occurred on November'SOth, on receipt 
I of some muscle forwarded by Major Percy, of Hodnet, and said 
• by him to have been taken from a typical case. A considerable 
quantity (4 cc.) of expressed muscle-juice was injected into 
