456 Report of Experimental Work on Protective 
the thighs of each of three vaccinated and three unvaccinated 
calves. Save slight lameness of two of the animals (one vacci- 
nated and one unvaccinated), they appeared to remain in perfect 
health, the thermometer indicating normal temperature for six 
following days. 
On January 7th, 1887, Mr. Couchman, V.S., of Wadhurst, 
telegraphed that he had met with a marked case of Quarter 111 
in a young bull still alive, and within two miles of Wadhurst 
Park. On the 8th, Mr, Penberthy proceeded by early train to 
the spot, recognized a clear case, obtained the necessary material 
from the affected muscles of the bull, which had died 18 hours 
previously, and injected 5 cc. into the thigh of each of the 
so far untested experimental animals, i.e. three vaccinated and 
three unvaccinated. Strangely enough, with the exception of a 
considerable local swelling in one of the vaccinated calves at 
the seat of inoculation, and the elevation of its temperature on 
the following morning to 106 "2°, there was no disturbance. 
The temperature of this animal dropped by degrees daily to 
normal. 
On return to College, a guinea-pig was inoculated with 
one drop of muscle-juice, and three rabbits with three drops 
each. The rabbits suffered no ill-effects, while the guinea-pig 
died of Quarter III in 28J hours. The principal inference 
from this experiment, one item of which entailed four journeys 
into the middle of Sussex, appears to be evidence in direction 
of the view that circumstances may materially modify the in- 
fecting power of the virus, or the susceptibility of the animal. 
To the fact of a mild attack of the naturally contracted disease 
establishing protection we shall again refer. In absence of 
satisfactory elucidation of the reason we must, at least for the 
time, be content to accept it as an axiom that extrinsic and 
intrinsic conditions affecting the animal, and probably the 
virus, obtain to a greater extent in this than in rr^any other 
contagious diseases. This bears out the result of clinical ob- 
servation generally as to its erratic occurrence. 
In order to give another trial to M. Arloing's " vaccine " 
powder, a fresh quantity was obtained from his agents in Paris, 
and on January 18th at the College, a heifer and steer, each 
about 10 months old, and a guinea-pig, were vaccinated in 
accordance with the directions with the powder. After the 
prescribed interval the process was completed. Careful obser- 
vation revealed no disturbance from the operation. 
On February 3rd, Mr. Godman of Woldingfold, Horsham, 
with whom we were previously in communication, wrote saying 
that one of his heifers was attacked with Quarter III, and thai 
he wished the remainder of his young stock " protected." Ac- 
