Inoculation for Anthrax and Quarter Til. 
457 
cordingly, on the following morning, Mr. Penberthy proceeded 
to Woldingfold, and " vaccinated " twenty-three animals with 
Arloing's No. 1 vaccine, intending to go down and finish the 
process in due course. The case of the heifer was characteristic, 
and some muscle-juice containing the virus in considerable 
numbers was used to test the effect of the powder on the vac- 
cinated steer and heifer at the College, and on a guinea-pig. 
In the case of the cattle a few drops were injected at 8.35 P.M. 
into the thigh of each, and one drop into the subcutaneous 
tissue of the guinea-pig. On the following morning the animals 
were noticeably ill. Symptoms of acute Quarter 111 developed ; 
the guinea-pig died 24^ hours, the steer 44^ hours, and the 
heifer 47 hours after inoculation. The following chart shows 
temperatures at : — 
4th Feb. 1SS7. Steer. Heifer. 
9a.m 104-3 Fabr 105-6 i'alir. 
2p.m 102-3 „ .. .. 106-4 „ 
9-30 P.M. .. 101-5 ,, .. .. 102-2 ,, 
Post-mortem examination revealed typical Quarter 111. After 
this, with the concurrence of Mr. Godman, it was decided that 
the remainder of the process of vaccination by Arloing's powder 
method should not be carried out on the 23 calves. 
At the same time that the heifer, steer, and guinea-pig were 
vaccinated with Arloing's powder, we inoculated another guinea- 
pig with muscle-juice dried at 32° C. prepared by ourselves. 
This little animal was subjected to precisely the same condi- 
tion of inoculation of virulent material from Godman's case as 
the calves and a guinea-pig, and experienced no ill-effect, living 
apparently in perfect health for two months after. 
This latter circumstance induced us to make further trials 
of material prepared on the principle of the last mentioned. 
Muscle-juice was dried and subjected to various temperatures. 
On February 14th, six guinea-pigs were vaccinated, three with 
powder produced at a temperature of 32° C, and three with 
some produced at 39° C. Forty-eight hours after inoculation 
one of the latter died, and 98 hours after one of those inoculated 
with 32° C. powder. 
On February 25th and March 7th, three fresh guinea-pigs 
were vaccinated with Arloing's powder. 
Up to the latter date the four surviving inoculated guinea- 
pigs appeared perfectly well. On the morning of the 8th of 
March one was found dead, which was inoculated with 32° C. 
material on February 14tli, — one which had been inoculated 
and, as above, resisted virulent injection. One by one they were 
found dead, until by the 4th of April the whole of our experi- 
