458 Report of Experimental Work on Protective 
mental animals had succumbed. During the occurrence of this 
mortality there was great variation of atmospheric temperature, 
in addition to which we now have reason for supposing there was 
neglect on the part of the special attendant, who was discharged 
at this date. Post-mortem examination yielded no evidence of 
Quarter III. 
These accidents were of course disastrous to this aspect of 
our work, which there had been reason for hoping might yield 
some practical result. We may, however, learn from it that 
three out of four guinea-pigs which had been inoculated with 
muscle-juice dried at 32° C, and two out of three inoculated 
with muscle-juice dried at 39° C, survived without manifesting 
ill-effects. It is also remarkable that one guinea-pig inoculated 
with muscle-juice dried at 32° C. suffered no inconvenience 
from injection of virulent matter, which killed two calves and 
a guinea-pig " vaccinated " with Arloing's powder. 
It should perhaps be mentioned here that the French experi- 
menters first dry the muscle-juice at 32° C, and then submit it 
— mixed with water — for some hours to dry heat of 100° C, 
and 85° C, respectively for " first and second vaccines." The 
effect of the high temperature is probably the attenuation of the 
virus. It seems to us highly probable that the system of the 
calf would resist the injurious effects of a more potent virus 
than that in use in the form of M. Arloing's powder, and that 
a more potent virus would be more likely to make a protective 
impression on its subject. We therefore think it desirable that 
further investigation into this matter should be prosecuted. 
Another phase of the disease is very interesting, and partly 
depending on it is the basis of another system of protective 
inoculation. The virus when introduced in moderate quanti- 
ties (a fraction of which into the muscles would be fatal) into 
the blood stream of a susceptible animal, is incapable of in- 
ducing the affection. It has been asserted, by the writers before 
referred to, that introduction of fresh virus into the blood current 
of the living animal does not induce the disease, but that it 
actually confers on the animal so treated immunity for periods 
ranging from one to two years. 
Experiment on this system was rendered practicable by the 
magnanimous offer of Major Algernon Heber Percy, of Hod net 
Mail, Shropshire, who communicated to us an expression of his 
great interest in the work in hand, and volunteered to provide 
the necessary animals and attention for carrying out a series of 
trials on this special point at his farm adjoining Hodnet Hall. 
The offer was cordially accepted, and the necessary preliminary 
arrangements made. Commencement of operations depended 
no the occurrence of a typical case of Quarter 111 at or near 
