Inoculation for Anthrax and Quarter HI. 
459 
Hodnet. The opportunity arose on Xovember 11th, when, in 
response to a telegram from Major Percv, Mr. Penberthy pro- 
I ceedeJ to Hodnet the same night. On arrival it was found that 
I the animal which was to provide the protective material had 
died at 11.45 A.'Sl. Major Percv, with the kind assistance of 
his neighbours. Viscount Hill, Sir Vincent Corbett, Sir Thomas 
Mevrick, Mr. J. Tayleur, Mr. J. Bibbj, and Rev. J. Hill, had 
collected early in November eight calves of different sizes, and 
of ages varying from 6 to 10 months. Thev had for some days 
been grazing together, and were now placed at our disposal for 
the experiment. It was decided that four of these animals 
should be protected by intravenous injection and placed with 
the remaining four ; the whole to be subject, save for inoculation, 
to precisely same conditions. To facilitate reference, the calves 
were marked and known as 1, 2, 3 and 4, and A, B, C and D. 
On the morning of Xovember 12th, a large number of 
agriculturists, veterinary surgeons and others had assembled to 
I witness the operation. Examination of the dead calf revealed a 
I clear case of Quarter 111, apparently free from putrefactive 
I change. Pieces of muscle from the most affected part were then 
taken and the juice obtained by squeezing through coarse linen. 
After diluting with water, the material for inoculation was ready. 
Calf No. 1 was then cast and secured with ropes, the jugular 
vein laid bare by dissection, and with the utmost precaution, to 
avoid contaminating the connective tissues, a few drops of the 
, diluted muscle-juice were injected into the blood stream by 
means of a very fine hypodermic svringe. No. 2 was treated 
in the same way. In the case of Nos. 3 and 4, the material 
was introduced into the jugular vein bv passing the hvpodermic 
I syringe point directly into it through the skin without previous 
dissection. Duly marked for identification, the eight calves were 
turned into a pasture and treated in every way alike, except that 
the temperatures of the inoculated were taken twice daily for 
eleven days. The register on page 460 shows that the heat- 
regulating functions were scarcely noticeably disturbed. The 
thermometry was undertaken by ^lajor Percv, who personally 
superintended it, and Mr. Tomes, bailiff", who evinced the greatest 
care and interest throughout, states that up to the 24th all the 
calves appeared perfectly well. 
I It was previously arranged that at the expiration of seven 
days the virtue of this intravenous inoculation should be tested 
by injection into the muscles of virulent matter obtained from 
a naturally occurring case of Quarter 111 ; the whole eight 
being subjected to the same treatment. To obtain the fresh 
virus for this purpose we put ourselves in communication with 
several stock-owners and veterinary surgeons. On receipt of 
